LOCUS       sequence-60680-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUMBLA
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             617..634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(687..705)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      701..748
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(872..891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggcta
      121 gccggccaca ccaatggcaa gctgacgaag atgctgttcg gaagggtacc tgctcctttc
      181 cggtccgcta cctgggtcat gtagaggtgg aggaatcgcg gggcatgcat gtttgtgagg
      241 acgcagtcaa aaagttaaaa gccatggggc gtaagtcagt caaaagtgtg ctgtgggttt
      301 cagcggatgg cctgcgcgtc gtggacgata aaaccaaaga tttactggta gaccagacga
      361 ttgaaaaggt ttccttctgc gccccagatc gtaacctgga caaagcattt tcgtatatct
      421 gtcgcgatgg tactacacgg cgttggattt gccactgttt cttagctctg aaagacagcg
      481 gggaacgcct gtctcacgcg gtgggatgcg cctttgcagc gtgtttagag cgtaagcagc
      541 gtcgcgaaaa atgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct
      601 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      661 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      721 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      781 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      901 gccacgttcg ccggctttcc ccgtca
//
