LOCUS       sequence-60682-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA6A
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggagc
      121 tcgcggtggg catcgacctg ggcaccacct actcgtgcgt gggcgtgttt cagcagggcc
      181 gcgtggagat cctggccaac gaccagggca accgcaccac gcccagctac gtggccttca
      241 ccgacaccga gcggctggtc ggggacgcgg ccaagagcca ggcggccctg aacccccaca
      301 acaccgtgtt cgatgccaag cggctgatcg ggcgcaagtt cgcggacacc acggtgcagt
      361 cggacatgaa gcactggccc ttccgggtgg tgagcgaggg cggcaagccc aaggtgcgcg
      421 tatgctaccg cggggaggac aagacgttct accccgagga gatctcgtcc atggtgctga
      481 gcaagatgaa ggagacggcc gaggcgtacc tgggccagcc cgtgaagcac gcagtgatca
      541 ccgtgcccgc ctatttcaat gactcgcagc gccaggccac caaggacgcg ggggccatcg
      601 cggggctcaa cgtgttgcgg atcatcaatg agcccacggc agctgccatc gcctatgggc
      661 tggaccggcg gggcgcggga gagcgcaacg tgctcatttt tgacctgggt gggggcacct
      721 tcgatgtgtc ggttctctcc attgacgctg gtgtctttga ggtgaaagcc actgctggag
      781 atacccacct gggaggagag gacttcgaca accggctcgt gaaccacttc atggaagaat
      841 tccggcggaa gcatgggaag gacctgagcg ggaacaagcg tgccctgcgc aggctgcgca
      901 cagcctgtga gcgcgccaag cgcaccctgt cctccagcac ccaggccacc ctgga
//
