LOCUS       sequence-60684-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT11A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgcggc
      121 cccagcccgg cgatggtgag cggcggtacc gagaggccag tgccagaaag aagatccgcc
      181 tggacaggaa gtacatcgtg tcctgtaagc agacagaggt gcccctctcc gtcccctggg
      241 accctagcaa ccaggtgtac ctgagctaca ataacgtctc ctccttgaag atgcttgtgg
      301 ccaaggacaa ctgggtgctg tcctcggaga tcagtcaggt ccgcctgtac actctggagg
      361 atgacaagtt cctctccttc cacatggaga tggtggtgca tgtggatgca gcccaggcct
      421 tcctgctgct ctcggacctg cgtcagaggc cagagtggga caagcactac cggagcgtgg
      481 agctagtgca gcaggtagac gaggacgacg ccatctacca cgtcaccagc cctgccctcg
      541 gaggtcacac aaagccccag gacttcgtga tcctggcctc gaggcggaag ccttgtgaca
      601 atggggaccc ctatgtcatc gcgctgaggt cggtcacgct gcccacacac cgagagacgc
      661 cagagtacag acgcggagag accctctgct caggcttctg cctctggcgc gagggggacc
      721 agctgaccaa ggtatcctac tacaaccagg ccaccccagg tgttctcaac tatgtgacca
      781 ccaacgtggc cggcctctcc tctgagttct acaccacctt caaggcttgt gagcagtttc
      841 tcttggacaa ccggaatgat ctggccccca gcctccagac cctctgacag taaaggtgga
      901 tacggatccg aattcgagct cgtc
//
