LOCUS       sequence-60685-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT6A
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             452..469
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(522..540)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      536..583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(707..726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggatc
      121 tgcagggcga gctggacaga ttcgggggca tctcggtgcg cctggcgcgg ctcgatgcgc
      181 tggaccgcct ggacgctgcc gccttccaga agggcttgca ggctgcagta cagcaatggc
      241 gatcagaagg tagaacagct gtatggctgc acattcccat cctccaaagc cgatttattg
      301 cccctgctgc ttccctgggc ttctgctttc accacgcaga atcggattca tcaacgttga
      361 ctctgtggct gagagaaggg cccagctgac agtaaaggtg gatacggatc cgaattcgag
      421 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      481 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      541 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      601 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      901 tccacgttct ttaatagtgg actctttgtt ccaaactgga cacactcacc tatctcggtc
//
