LOCUS       sequence-60688-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA1LA
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggcta
      121 ctgccaaggg aatcgccata ggcatcgacc tgggcaccac ctactcctgt gtgggggtgt
      181 tccagcacgg caaggtggag atcatcgcca acgaccaggg caaccgcacc acccccagct
      241 acgtggcctt cacagacacc gagcggctca ttggggatgc ggccaagaac caggtagcaa
      301 tgaatcccca gaacactgtt tttgatgcta aacgtctgat cggcaggaaa tttaatgatc
      361 ctgttgtaca agcagatatg aaactttggc cttttcaagt gattaatgaa ggaggcaagc
      421 ccaaagtcct tgtgtcctac aaaggggaga ataaagcttt ctaccctgag gaaatctctt
      481 cgatggtatt gactaagttg aaggagactg ctgaggcctt tttgggccac cctgtcacca
      541 atgcagtgat taccgtgcca gcctatttca atgactctca acgtcaggct actaaggatg
      601 caggtgtgat tgctggactt aatgtgctaa gaatcatcaa tgagcccacg gctgctgcca
      661 ttgcctatgg tttagataaa ggaggtcaag gagaacgaca tgtcctgatt tttgatctgg
      721 gtggaggcac atttgatgtg tcaattctga ccatagatga tgggattttt gaggtaaagg
      781 ccactgctgg ggacactcac ctgggtgggg aggactttga caacaggctt gtgagccact
      841 tcgtggagga gttcaagagg aaacacaaaa aggacatcag ccagaacaag cgagccgtga
      901 ggcggctgcg caccgcctgc gagagggcca agaggaccct gtcgtccagc acccaggc
//
