LOCUS       sequence-60690-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NXR1A
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      728..775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(899..918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgtcgg
      121 cgccggccgg ggagccgccg gcgcccgtgc ggctgcggaa gaacgtgtgc tacgtggtgc
      181 tggccgtgtt cctcagcgag caggatgagg tgctactgat ccaggaggcc aagagggagt
      241 gccgggggtc gtggtacctg cctgcgggga gaatggagcc aggggagacc atcgtggagg
      301 cgctgcagcg ggaggtgaag gaggaggcgg ggctgcactg tgagcccgag acactgctgt
      361 ccgtggagga gcggggcccc tcctgggtcc gcttcgtgtt cctcgctcgc cccacaggtg
      421 gaattctcaa gacttccaag gaggccgatg cggagtccct gcaggctgcc tggtacccac
      481 ggacctccct gcccactccg ctgcgagccc atgacatcct gcacctggtt gaactagccg
      541 cccagtatcg ccagcaagcc aggcaccctc tcattctgta acagtaaagg tggatacgga
      601 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      661 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gc
//
