LOCUS       sequence-60712-e        5169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ909
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(495..512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(495..511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(517..528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3173..3195
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3233..3251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3319..3423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3424..3492,3493..4284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3642..3661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4455..5043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4944..4963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctacgt aatacgactc actagtgggc agatcttcga atgcatcgcg
      361 cgcaccgtac gtctcgagga attcctgcag gatatctgga tccacgaagc ttcccatggt
      421 gacgtcaccg gttctagata cctaggtgag ctctggtacc ctctagtcaa ggccttaagt
      481 gagtcgtatt acggactggc cgtcgtttta catggcacgc ccttctatta cagggtttct
      541 gataacaaac cggttattaa ctcccaacaa gggatgattt caattcatca tgctcaaatt
      601 gacccaaatt aagttacatg acaaattcat cgcccttttc aactcttctt ggctcatcat
      661 ctgttattct gttcctattc tgtctgcacc ctatacccct ttgcatactc tctcgtcatt
      721 cctttcttgg atagtgtctt cttctcccca gctctgctac ttctatgact tgcgcccgct
      781 gcttttccgc tcgttcctct cctctctgac gtcttcttct gcttcttttc tccttccttt
      841 ttcccttctc aatcttcctc tttttcccat ttctgtcaat cattcgaaga agaagaagaa
      901 gaagaccctc attcatttca tttttttttc tgtcggtgtg tgctgctcgg ttaagagtga
      961 gctctctatt ccacgtcttc ttctttttct tcttcgattc gaatcaatca ctccaccaaa
     1021 acgcattcgt ttttgggatt caccccgcgg ttcgcaatag gtttcttttt caaattatta
     1081 gcgttataaa tagaaaaatg gtggagtttc aataaaaatg ataatttaca aagtgtattt
     1141 tgattacatg tactcaaaaa ggttgaaatt tttcatacca gttttccgga aatccatctg
     1201 atatcattat cgtatttttc tttttaaaaa tgttttcaaa aaaaaacaaa atatagctgg
     1261 ttatttggca ccctctaatt accattttcc tgtcacacca cactcttttc cttcttccac
     1321 ttctttccgt tttcagccgc ttccaaccaa accgatatga aagccgagca acaacgatcg
     1381 attccacccg gctcggcgac cttcccgtcg caggtgagac tcagaaaact agagaaaccg
     1441 ctcaactaac tcttgatatc cgatttcatt cttttctctt ttctttttgt tgaacttttc
     1501 catatttcca gatgccacgg ccaccaccaa gcaccgaaca aggaatcaca acggaatcgg
     1561 agcttgcgaa gaaagctcaa atcactccga acgacgtttt agcacttccg ggaatcactc
     1621 aaggtatctt gctccctttg gatttcagtt taaaacataa atttaggatt cttatgctcc
     1681 ccatctgcga acatctataa catcgagttc accaagttcc aaatccgtga tctggacact
     1741 gagcaagtgc ttttcgagat cgccaaaccg gagaacgatc aggagaatga tgagtcgcca
     1801 caggagtcgg caagatacgt gcgttataga tttgctccaa actttttgaa actcaaaacg
     1861 gtcggagcaa ctgtggaatt caaagtagga gacatcccaa tccatcattt ccgaatgatc
     1921 gaacgtcact tcttcaaaga tcgccttctg aagtgttttg actttgaatt cggattctgt
     1981 attccgaatt cacgaaacaa ctgtgaacat atctatgagt tccctcaact ctctcaacaa
     2041 ctcagtgagt cattattcta aaaagtacaa ttcaaaagac taatctcttt ttcagtggac
     2101 gacatgatca acaatccaaa cgagactcgt tctgacagct tctatttcgt cgataacaaa
     2161 ctcgtcatgc acaacaaagc cgactactca tatgatgcat aaatatttaa tacaaaaatg
     2221 ttctggataa ttattctgtc gaatagaaaa aaaactccaa atgtgattaa attccaataa
     2281 ttcctgtcta gtttgttcct tccccttccc cttctcatgt tcaatgcatt cctaagcttt
     2341 tcagttcccc ccttgtttct atattttttc gctgtcctgt cacactcgct aaaaacacta
     2401 atcacacgga aatctgtttt caataaaaac tccaacttta actcattttc aatttcaact
     2461 gaaagatttt ttcattagag aatgtctaga actaggcccg ggctacgtaa tacgactcac
     2521 ttaagggtgc cggatctgcg gcttactcac cagacctggc cccatccgat taccacctat
     2581 tcgcttcgat gggacacgca ctcgctgagc agcgcttcga ttcttacgaa agtgtgaaaa
     2641 aatggctcga tgaatggttc gccgcaaaag acgatgagtt ctactggcgt ggaatccaca
     2701 aattgcccga gagatgggaa aaatgtgtag ctagcgacgg caaatacttt gaataaatga
     2761 ttttttcttt ttccacaaaa tttaacgtgt tttttgattt aaaaaaaacg acatttcata
     2821 cttgtacacc tgatatctag atacctaggt gagctctggt acccccctgg cgttacccaa
     2881 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2941 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     3001 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtgcaatc
     3061 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3121 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3181 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3241 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3661 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4561 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     4621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct tttgctggcc ttttgctcac
     5101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5161 gctgatacc
//
