LOCUS       sequence-60725-e        5716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ496
FEATURES             Location/Qualifiers
     source          1..5716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1258..1305)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(2009..2020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3997..4018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4269..4657
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     promoter        4751..4855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4856..4924,4925..5716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNVGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5074..5093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cccagcccgc ctaatgagcg ggcttttttt tgaacaaaag gctgcagctg gacttccagc
       61 ggatgaatgg cagaaattcg atgataagct gtcaaacatg agaattggtc gacggcccgg
      121 gcggccgcaa ggggttcgcg ttggccgatt cattaatgca gctggcacga tccccggcga
      181 aacggtgaat acggcacgct accaacaaca attgatcaat ttgaaccgtg cgcttcagag
      241 aaaacgaccg gaatatcaaa aaagacaaca cagggtcatt tttctccatg acaacgctcc
      301 atcacatacg gcaagagcgg ttcgcgacac gttggaaaca ctcaattggg aagtgcttcc
      361 gcatgcggct tactcaccag acctggcccc atccgattac cacctattcg cttcgatggg
      421 acacgcactc gctgagcagc gcttcgattc ttacgaaagt gtgaaaaaat ggctcgatga
      481 atggttcgcc gcaaaagacg atgagttcta ctggcgtgga atccacaaat tgcccgagag
      541 atgggaaaaa tgtgtagcta gcgacggcaa atactttgaa taaatgattt tttctttttc
      601 cacaaaattt aacgtgtttt ttgatttaaa aaaaacgaca tttcatactt gtacacctga
      661 tacaactttc gggcacggca tggacatgta cagtgcctgc tcgccgtcag aggcagtgcc
      721 atcatacagg gcagtgtcca ggccgcagag agtaaagccc atgcggcggt atgcgtggat
      781 agccggtgcg ttgacgttgg tcacttcgag ccacaggtgg ccagcaccac gttcacgagc
      841 aaactcggta gccagaccca tgagtgcgcg accaacacca tgaccacggt gttccggtgc
      901 cacctcgatg tcttccacag tcagacggcg gttccagcca gagtagctca cgaccacgaa
      961 accagccagg tcgccgtcat caccataggc cacgaaggtg cgagagtctg gatcaccgtc
     1021 ttcgccgtcg tcggattcgt cgtcggattc gtcgtccggg aacactttgg tcagcggtgg
     1081 gtccactggc acttcacgca gggtgaaacc atcgccggtt gcagtgacgc gaaagacggt
     1141 gtcagtggtg aaggagccgt ccagagcctc gattgcttca gcgtcacctg ggacagaggt
     1201 gcggtagcga tatgcggtgt cgtcgagggt agtgcccatg gtttagttcc tcaccttgtc
     1261 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgtgggtag
     1321 ggataacagg gtaataaagt tgcccgggct atcaggtgta caagtatgaa atgtcgtttc
     1381 gaacatatag atgtctcgca aacgtaaata tttatcgatt gtcataaaac tttgaccttg
     1441 tgaagtgtca accttgactg tcgaaccacc atagtttggc gcgaattgag cgtcataatt
     1501 gtttactctc agtgcagtca acatgtcgag tttcgtgccg aataaagagc aaacgcggac
     1561 agtattaatt ttctgttttc atttgaagaa aacagctgcg gaatcgcacc gaatgcttgt
     1621 tgaagccttt ggcgaacaag taccaactgt gaaaacgtgt gaacggtggt ttcaacgctt
     1681 caaaagtggt gattttgacg tcgacgacaa agagcacgga aaaccgccaa aaaggtacga
     1741 agacgccgaa ctgcaagcat tattggatga agacgatgct caaacgcaaa aacaactcgc
     1801 agagcagttg gaagtaagtc aacaagcagt ttccaatcgc gatcccatcc tatgcttgca
     1861 ctttgagcca atttatccaa gtccttgtaa aaagtattcg aaaattgtta acggataaaa
     1921 tgtttattat aatatcaaaa acaatttgtc agttgaccac tttttgatat atttgacaga
     1981 aaccgggatg aattggctca aagttagggc gcccttctat tacagggttt ctgataacaa
     2041 accggttatt aactcccaac aagggatgat ttcaattcat catgctcaaa ttgacccaaa
     2101 ttaagttaca tgacaaattc atcgcccttt tcaactcttc ttggctcatc atctgttatt
     2161 ctgttcctat tctgtctgca ccctataccc ctttgcatac tctctcgtca ttcctttctt
     2221 ggatagtgtc ttcttctccc cagctctgct acttctatga cttgcgcccg ctgcttttcc
     2281 gctcgttcct ctcctctctg acgtcttctt ctgcttcttt tctccttcct ttttcccttc
     2341 tcaatcttcc tctttttccc atttctgtca atcattcgaa gaagaagaag aagaagaccc
     2401 tcattcattt catttttttt tctgtcggtg tgtgctgctc ggttaagagt gagctctcta
     2461 ttccacgtct tcttcttttt cttcttcgat tcgaatcaat cactccacca aaacgcattc
     2521 gtttttggga ttcaccccgc ggttcgcaac aggtttcttt ttcaaattat tagcgttata
     2581 aatagaaaaa tggtggagtt tcaataaaaa tgataattta caaagtgtat tttgattaca
     2641 tgtactcaaa aaggttgaaa tttttcatac cagttttccg gaaatccatc tgatatcatt
     2701 atcgtatttt tctttttaaa aatgttttca aaaaaaaaca aaatatagct ggttatttgg
     2761 caccctctaa ttaccatttt cctgtcacac cacactcttt tccttcttcc acttctttcc
     2821 gttttcagcc gcttccaacc aaaccgatat gaaagccgag caacaacaat cgattccacc
     2881 cggctcggcg accttcccgt cgcaggtgag actcagaaaa ctagagaaac cgctcaacta
     2941 actcttgata tccgatttca ttcttttctc ttttcttttt gttgaacttt tccatatttc
     3001 cagatgccac ggccaccacc aagcaccgaa caaggaatca caacggaatc ggagcttgcg
     3061 aagaaagctc aaatcactcc gaacgacgtt ttagcacttc cgggaatcac tcaaggtatc
     3121 ttgctccctt tggatttcag tttaaaacat aaatttagga ttcttatgct ccccatctgc
     3181 gaacatctat aacatcgagt tcaccaagtt ccaaatccgt gatctggaca ctgagcaagt
     3241 gcttttcgag atcgccaaac cggagaacga tcaggagaat gatgagtcgc cacaggagtc
     3301 ggcaagatac gtgcgttata gatttgctcc aaactttttg aaactcaaaa cggtcggagc
     3361 aactgtggaa ttcaaagtag gagacatccc aatccatcat ttccgaatga tcgaacgtca
     3421 cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa ttcggattct gtattccgaa
     3481 ttcacgaaac aactgtgaac atatctatga gttccctcaa ctctctcaac aactcagtga
     3541 gtcattattc taaaaagtac aattcaaaag actaatctct ttttcagtgg acgacatgat
     3601 caacaatcca aacgagactc gttctgacag cttctatttc gtcgataaca aactcgtcat
     3661 gcacaacaaa gccgactact catatgatgc ataaatattt aatacaaaaa tgttctggat
     3721 aattattctg tcgaatagaa aaaaaactcc aaatgtgatt aaattccaat aattcctgtc
     3781 tagtttgttc cttccccttc tccttctcat gttcaatgca ttcctaagct tttcagttcc
     3841 ccccttgttt ctatattttt tcgctgtcct gtcacactcg ctaaaaacac taatcacacg
     3901 gaaatctgtt ttcaataaaa actccaactt taactcattt tcaatttcaa ctgacaggtt
     3961 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4021 ggcaccccag gctttacatg gggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     4081 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     4141 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa taaggatgga tataccgaaa
     4201 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaacggccac gatgcgtccg
     4261 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     4321 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     4381 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     4441 taagctctca tgtttgaaca ataaaattaa tataaatcag cgacttaaat agcctctaag
     4501 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     4561 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     4621 caattttgag tgacacagga acacttaacg gctgacatgg gaattagctt cacgctgccg
     4681 caagcactca gggcgcaagg gctgctaaag gaagcggata gacgtaggtg gcacttttcg
     4741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     5101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5281 accgaaggag ctaaccgctt ttttgcacaa cgtgggggat cacgtaactc gccttgatcg
     5341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     5401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5701 gattaagcat tggtaa
//
