LOCUS       sequence-60869-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: pBOB-CAG-fHis-H2A-Ub
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    25..146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(36..55)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     90..109
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(276..650)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(669..716)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(832..851)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa accacaacta
       61 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      121 ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg
      181 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
      241 aaggcggcgg tggaatcgaa atctcgtagc acgtgtcagt cctgctcctc ggccacgaag
      301 tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg
      361 atctcggtca tggccggccc ggaggcgtcc cggaagttcg tggacacgac ctccgaccac
      421 tcggcgtaca gctcgtccag gccgcgcacc cacacccagg ccagggtgtt gtccggcacc
      481 acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac accggcgaag
      541 tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg tccagaactc gaccgctccg
      601 gcgacgtcgc gcgcggtgag caccggaacg gcactggtca acttggccat ggtttagttc
      661 ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt
      721 gctgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc
      781 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct
      841 ctgcataaat aaaaaaaatt agtcagccat gggggcgg
//
