LOCUS       sequence-60878-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKW32 converted Gateway Destination vector for expressing hemoglobin
            tagged fusion proteins in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYHb
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(502..626)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(636..653)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gatacagtag
      121 aaattacaga aactttatca cgtttagtaa gtatagaggc tgaaaatcca gatgaagccg
      181 aacgacttgt aagagaaaag tataagagtt gtgaaattgt tcttgatgca gatgattttc
      241 aggactatga cactagcgta tatgaatagg tagatgtttt tattttgtca cacaaaaaag
      301 aggctcgcac ctctttttct tatttctttt tatgatttaa tacggcattg aggacaatag
      361 cgagtaggct ggatacgacg attccgtttg agaagaacat ttggaaggct gtcggtcgac
      421 taagttggca gcatcacccg aagaacattt ggaaggctgt cggtcgacta caggtcacta
      481 ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca gtattatgta
      541 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      601 cgttcagctt ttttgtacaa acttgtatta tgttcactcc ctcgagggac caagcctccg
      661 cctccgcctg atattcctgg ttcaaccgct tgagcgtaca aatctgcttc cacttgaata
      721 aacacatctg caatcacgcc ataagccttg ccccacgcgt ccaaaatgtc atcgg
//
