LOCUS       sequence-60879-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEG202 converted Gateway Destination vector for yeast two-hybrid 
            bait vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY202
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(498..622)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(676..697)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgata cagtagaaat
      121 tacagaaact ttatcacgtt tagtaagtat agaggctgaa aatccagatg aagccgaacg
      181 acttgtaaga gaaaagtata agagttgtga aattgttctt gatgcagatg attttcagga
      241 ctatgacact agcgtatatg aataggtaga tgtttttatt ttgtcacaca aaaaagaggc
      301 tcgcacctct ttttcttatt tctttttatg atttaatacg gcattgagga caatagcgag
      361 taggctggat acgacgattc cgtttgagaa gaacatttgg aaggctgtcg gtcgactaag
      421 ttggcagcat cacccgaaga acatttggaa ggctgtcggt cgactacagg tcactaatac
      481 catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct
      541 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
      601 cagctttttt gtacaaactt gtattaatga attccagcca gtcgccgttg cgaataaccc
      661 caaccgccag cccttcaatg gtgaagctct gctgacgaag atctacgaca attggtttaa
      721 actcgctatt ttctggcaac agttcgactt tattgccctg ttttttcagg cgcttaacgg
      781 taac
//
