LOCUS       sequence-60880-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEG202 converted Gateway Destination vector for yeast two-hybrid 
            bait vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY202
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..184
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        362..464
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(531..550)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaatcataa atcataagaa attcgcccgg aattagcttg gctgcaggtc gactcgagga
       61 ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      121 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      181 tatgaatcaa ctacttagat ggtattagtg acctgtagtc gactaagttg gcagcatcac
      241 ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
      301 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
      361 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
      421 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      481 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      541 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      601 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      661 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      721 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      781 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      841 g
//
