LOCUS       sequence-60884-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Lee JH, Silhavy JL, Lee JE, Al-Gazali L, Thomas S, Davis EE, Bielas 
            SL, Hill KJ, Iannicelli M, Brancati F, Gabriel SB, Russ C, Logan CV,
            Sharif SM, Bennett CP, Abe M, Hildebrandt F, Diplas BH, Attie-Bitach
            T, Katsanis N, Rajab A, Koul R, Sztriha L, Waters ER, Ferro-Novick 
            S, Woods CG, Johnson CA, Valente EM, Zaki MS, Gleeson JG
  TITLE     Evolutionarily assembled cis-regulatory module at a human ciliopathy
            locus.
  JOURNAL   Science. 2012 Feb 24;335(6071):966-9. Epub 2012 Jan 26.
  PUBMED    22282472
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2012-02-24"; volume: "335(6071)"; pages: "966-9. Epub 2012 
            Jan 26"
COMMENT     Sequence Label: TMEM216-mCherry
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..713)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(281..300)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     550..568
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agctacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
       61 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      121 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      181 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      241 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      301 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
      361 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      421 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
      481 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
      541 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      601 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
      661 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catacgcgta
      721 atcgtgtcca tactggagaa agcagccaag gtgagcacct caagtaaaag ctctgagcca
      781 cagaagaaga gcaagatgcc attcatgatg gcttccaggc ggagtacgta ggtctgcagc
      841 agcaggtaat aggaggccat catggcagat gggaa
//
