LOCUS       sequence-60891-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: FU-MAP2-Gateway
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(84..90,91..108,109..114))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(139..263)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(294..313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(295..313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
       61 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
      121 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
      181 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      241 tcagcttttt tgtacaaact tgttgatcaa gcttacctag ccagcttggg tctccctata
      301 gtgagtcgta ttactcgagt cggttagaga caagctgaag aatctaccga aacaaaacaa
      361 aagaaacata tatatatata tatatatgga tttgttaact aaatatcaaa aattataact
      421 caccagtaag caaatgcatt ctttatactc aaataataaa tctatgcctg gaggtctgta
      481 gcacaaaaaa aatgcagtac ttgaatattt aggaagctca ataatgaatt ttaaaagatc
      541 tttactctga gattacataa agcacatcct atgaaaaacg aattgaaaat gaaacgaaat
      601 aaaattaaaa gccccaaacc attaccagcg catatgcagc aataccactc aagtctagcc
      661 tttgagaact gctgcagaat ccttctccgg gagcattatc cccacaggct ggctctctgg
      721 ctctctagct ccagacagac gcagtctgca agccaccag
//
