LOCUS       sequence-60919-e       15497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, 
            Karunairetnam S, Gleave AP, Laing WA
  TITLE     Transient expression vectors for functional genomics, quantification
            of promoter activity and RNA silencing in plants.
  JOURNAL   Plant Methods. 2005 Dec 18;1:13.
  PUBMED    16359558
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2005-12-18"; volume: "1"; pages: "13"
COMMENT     Sequence Label: pHEX2
FEATURES             Location/Qualifiers
     source          1..15497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4..128
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(153..158,159..176,177..183)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             237..896
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(303..322)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1238..1543
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1422..1441
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1584..1708)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1748..2454
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(2495..2513)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      2542..2551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2581..2600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2582..2600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2607..2624)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2607..2623)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2616..2638)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2781..2964
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2965..3006,3007..3015,3016..3786)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3046..3065)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3656..3675
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4411..4663
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4726..4750
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            5304..5413
                     /note="incP origin of transfer"
     mobile_element  5473..6240
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             6865..8013
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(9202..9219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9373..9961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9453..9472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11058..11846
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      12521..13155
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    13640..13664
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13920..13941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13956..13961,13962..13979,13980..13986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13994..14010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13999..14021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14018..14034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14018..14034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        14052..14070
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        15130..15475
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     15418..15438
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
       61 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      121 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
      181 atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa gctaaaatgg
      241 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      301 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      361 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
      421 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
      481 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
      541 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
      601 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
      661 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
      721 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
      781 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
      841 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
      901 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
      961 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     1021 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     1081 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     1141 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     1201 aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta
     1261 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     1321 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     1381 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     1441 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     1501 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     1561 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     1621 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     1681 ctcgttcagc tttcttgtac aaagtggtga tgggttcgaa atcgataagc ttggatcctc
     1741 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
     1801 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
     1861 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
     1921 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
     1981 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
     2041 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
     2101 actgattaca taaatcttat tcaaatttca aaaggcccca ggggctagta tctacgacac
     2161 accgagcggc gaactaataa cgttcactga agggaactcc ggttccccgc cggcgcgcat
     2221 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
     2281 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
     2341 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
     2401 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg
     2461 caggcatgca agctagctta ctagtgatgc atattctata gtgtcaccta aatctgcggc
     2521 cgcactagtg atatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg
     2581 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2641 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2701 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2761 tggaaattgt aaacgttaat gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     2821 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     2881 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
     2941 cactctcaat ccaaataatc tgcaatggca attaccttat ccgcaacttc tttacctatt
     3001 tccgcccgga tccgggcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3061 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3121 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3181 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3241 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3301 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3361 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3421 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3481 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3541 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3601 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3661 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3721 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3781 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3841 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3901 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg
     3961 atccaacact tacgtttgca acgtccaaga gcaaatagac cacgaacgcc ggaaggttgc
     4021 cgcagcgtgt ggattgcgtc tcaattctct cttgcaggaa tgcaatgatg aatatgatac
     4081 tgactatgaa actttgaggg aatactgcct agcaccgtca cctcataacg tgcatcatgc
     4141 atgccctgac aacatggaac atcgctattt ttctgaagaa ttatgctcgt tggaggatgt
     4201 cgcggcaatt gcagctattg ccaacatcga actacccctc acgcatgcat tcatcaatat
     4261 tattcatgcg gggaaaggca agattaatcc aactggcaaa tcatccagcg tgattggtaa
     4321 cttcagttcc agcgacttga ttcgttttgg tgctacccac gttttcaata aggacgagat
     4381 ggtggagtaa agaaggagtg cgtcgaagca gatcgttcaa acatttggca ataaagtttc
     4441 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4501 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     4561 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     4621 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaattaa ttcagtacat
     4681 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     4741 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     4801 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     4861 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     4921 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     4981 tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct
     5041 cgcttaaccg tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg
     5101 gataaagccg ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatgt
     5161 cgacggatct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     5221 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     5281 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     5341 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     5401 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc
     5461 aagccaacca ggggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     5521 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     5581 gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac
     5641 atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga
     5701 tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa
     5761 cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg
     5821 ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa
     5881 tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac
     5941 gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt
     6001 gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag
     6061 ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg
     6121 cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat
     6181 gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc
     6241 caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga
     6301 ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc
     6361 agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca
     6421 atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca
     6481 cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg
     6541 acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt
     6601 agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc
     6661 aagaagagcg acttcgcgga gctggtattc gtgcagggca agattcggaa taccaagtac
     6721 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
     6781 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
     6841 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
     6901 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
     6961 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
     7021 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
     7081 gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
     7141 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
     7201 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
     7261 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
     7321 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
     7381 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
     7441 ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc
     7501 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
     7561 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
     7621 atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
     7681 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
     7741 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
     7801 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
     7861 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
     7921 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
     7981 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
     8041 ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact
     8101 tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag
     8161 gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc
     8221 aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg
     8281 tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg
     8341 tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg
     8401 acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc
     8461 gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga
     8521 tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac
     8581 ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg
     8641 tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc
     8701 taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg
     8761 cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg
     8821 cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc
     8881 ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag
     8941 ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt
     9001 ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac
     9061 tcgaacctac agttgtttcc ttactgggct ttctcagccg ggatggcgct aagaagctat
     9121 tgccgccgat cttcatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9181 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9541 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     9601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9781 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatatca
     9961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10261 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10381 acaagtggca gcaacggatt cgcaaacctg tcacgccttt tgtgccaaaa gccgcgccag
    10441 gtttgcgatc cgctgtgcca ggcgttaggc gtcatatgaa gatttcggtg atccctgagc
    10501 aggtggcgga aacattggat gctgagaacc atttcattgt tcgtgaagtg ttcgatgtgc
    10561 acctatccga ccaaggcttt gaactatcta ccagaagtgt gagcccctac cggaaggatt
    10621 acatctcgga tgatgactct gatgaagact ctgcttgcta tggcgcattc atcgaccaag
    10681 agcttgtcgg gaagattgaa ctcaactcaa catggaacga tctagcctct atcgaacaca
    10741 ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc gaatttgcga
    10801 aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa acgaacaatg
    10861 tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt gacctgttca
    10921 cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac tggttctcgg
    10981 gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg cgcggcttaa
    11041 ttcaggagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
    11101 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
    11161 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
    11221 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
    11281 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
    11341 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
    11401 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
    11461 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
    11521 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
    11581 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
    11641 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
    11701 gcctgccggc ccagtatcag cccgtcatac ttgaagctag gcaggcttat cttggacaag
    11761 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg
    11821 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
    11881 cggcgcggct taactcaagc gttagagagc tggggaagac tatgcgcgat ctgttgaagg
    11941 tggttctaag cctcgtactt gcgatggcat cggggcaggc acttgctgac ctgccaattg
    12001 ttttagtgga tgaagctcgt cttccctatg actactcccc atccaactac gacatttctc
    12061 caagcaacta cgacaactcc ataagcaatt acgacaatag tccatcaaat tacgacaact
    12121 ctgagagcaa ctacgataat agttcatcca attacgacaa tagtcgcaac ggaaatcgta
    12181 ggcttatata tagcgcaaat gggtctcgca ctttcgccgg ctactacgtc attgccaaca
    12241 atgggacaac gaacttcttt tccacatctg gcaaaaggat gttctacacc ccaaaagggg
    12301 ggcgcggcgt ctatggcggc aaagatggga gcttctgcgg ggcattggtc gtcataaatg
    12361 gccaattttc gcttgccctg acagataacg gcctgaagat catgtatcta agcaactagc
    12421 ctgctctcta ataaaatgtt aggagcttgg ctgccatttt tggggtgagg ccgttcgcgg
    12481 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    12541 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc cagggcgcag ccctggttaa
    12601 aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga
    12661 aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg
    12721 tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc
    12781 ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc
    12841 ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg
    12901 ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc
    12961 cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt
    13021 tttccgcgag gtatccacaa cgccggcggc cggccgcggt gtctcgcaca cggcttcgac
    13081 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    13141 agcccggtga gcgtcggaaa gggtcgacat cttgctgcgt tcggatattt tcgtggagtt
    13201 cccgccacag acccggattg aaggcgagat ccagcaactc gcgccagatc atcctgtgac
    13261 ggaactttgg cgcgtgatga ctggccagga cgtcggccga aagagcgaca agcagatcac
    13321 gattttcgac agcgtcggat ttgcgatcga ggatttttcg gcgctgcgct acgtccgcga
    13381 ccgcgttgag ggatcaagcc acagcagccc actcgacctt ctagccgacc cagacgagcc
    13441 aagggatctt tttggaatgc tgctccgtcg tcaggctttc cgacgtttgg gtggttgaac
    13501 agaagtcatt atcgtacgga atgccagcac tcccgagggg aaccctgtgg ttggcatgca
    13561 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg ttattctaat
    13621 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    13681 actgaaggcg ggaaacgaca atctgatcat gagcggagaa ttaagggagt cacgttatga
    13741 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgat
    13801 tgaaggagcc actcagcccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    13861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    13921 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    13981 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    14041 tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg
    14101 agctctccca tatcgacctg caggcggccg ctcgacgaat taattccaat cccacaaaaa
    14161 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
    14221 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
    14281 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
    14341 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
    14401 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
    14461 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
    14521 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
    14581 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
    14641 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
    14701 gcaggtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
    14761 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
    14821 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
    14881 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
    14941 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tgaagactgg
    15001 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
    15061 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagacca
    15121 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
    15181 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
    15241 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
    15301 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
    15361 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
    15421 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
    15481 gaggaattcg gtacccc
//
