LOCUS       sequence-60920-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged SIRT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H,
            Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP,
            Gygi SP, Sinclair DA, Alt FW, Greenberg ME
  TITLE     Stress-dependent regulation of FOXO transcription factors by the 
            SIRT1 deacetylase.
  JOURNAL   Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19.
  PUBMED    14976264
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-03-26"; volume: "303(5666)"; pages: "2011-5. Epub 2004 
            Feb 19"
COMMENT     Sequence Label: Flag-SIRT1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     246..268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     260..277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     261..277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     284..303
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        284..302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
       61 ggaggttttt taaagcaagt aaaacctcta cagatgtgat atggctgatt atgatcatta
      121 cttatctagg tataggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      241 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
      301 gggcgaattg aatttagcgg ccgcgaattc gcccttgtct agagtggaac aattcctgta
      361 cctgcacaat tattacacta tgatttgttt gatggatagt tcatgtctgt tacttcctgt
      421 ttcacagata tagcttcatt aattgcctct tgatcatctc catcagtccc aaatccagct
      481 cctccagctc tctctggaac atcaggctca tcttctaagc cattgtagaa ttcttcaatt
      541 tcactttcat cctccatggg ttcttctaaa cttggactct ggcatgtccc actatcactg
      601 ttactgccac aagaactaga ggataagacg tcatcttcag agtctgaata tacctcagcg
      661 ccatggaaaa tgtaacgatt tggtggcaaa aacagatact gattaccatc aagccgccta
      721 ctaatctgct cctttgccac tctattaggc cagcattttc tcactgttcc agccactgaa
      781 gttctttcat ttttctcccc agtactagaa ccaacattct tcaaatccgg attttccatc
      841 tgttcagcaa tactttcaac attcctagaa gtttgtactt cctgtggttt ttcttccata
      901 caaccttttg attcagacac atctaaatca
//
