LOCUS       sequence-60923-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a gRNA that targets Can1.Y in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p426-SNR52p-gRNA.CAN1.Y-SUP4t
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..216
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tttcattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
       61 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
      121 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
      181 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
      241 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
      301 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
      361 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
      421 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
      481 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
      541 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
      601 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
      661 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
      721 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
      781 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
      841 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
      901 gacattatta ttgttggaag aggactat
//
