LOCUS       sequence-60929-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Synthetic_Human_H2AZ.1
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             450..467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(520..538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      534..581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(705..724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     915..936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatggcg ggcggtaaag
       61 cgggtaaaga ttcgggcaaa gcaaaaacca aagccgtgag ccgtagccaa cgtgcgggtc
      121 tgcaatttcc ggtgggtcgt attcatcgcc acctgaaaag ccgtaccacg tctcatggtc
      181 gtgtgggtgc aaccgcagca gtttatagtg cagctatcct ggaatacctg acggccgaag
      241 tcctggaact ggcgggtaac gcctccaaag atctgaaagt gaaacgtatt accccgcgtc
      301 acctgcagct ggcaatccgt ggcgatgaag aactggacag cctgattaaa gcaacgatcg
      361 ctggcggtgg cgttatcccg cacattcaca aaagcctgat tggcaaaaaa ggtcaacaaa
      421 aaacggtctg ataagcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      481 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      541 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      601 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      901 cacgttcttt aatagtggac tcttgttcca aactgga
//
