LOCUS       sequence-60939-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC
  TITLE     IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary 
            epithelial cell transformation.
  JOURNAL   Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. 
            Epub 2012 Sep 24.
  PUBMED    23007157
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00468-12"; journalName: "Mol Cell Biol"; date: 
            "2012-12"; volume: "32"; issue: "23"; pages: "4756-68"
COMMENT     Sequence Label: pWB-Flag-IKKe
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttgactac aaagacgatg acgacaagca attgacaagt ttgtacaaaa aagttggcat
       61 gcagagcaca gccaattacc tgtggcacac agatgacctg ctggggcagg gggccactgc
      121 cagtgtgtac aaggcccgca acaagaaatc cggagagctg gttgctgtga aggtcttcaa
      181 cactaccagc tacctgcggc cccgcgaggt gcaggtgagg gagtttgagg tcctgcggaa
      241 gctgaaccac cagaacatcg tcaagctctt tgcggtggag gagacgggcg gaagccggca
      301 gaaggtactg gtgatggagt actgctccag tgggagcctg ctgagtgtgc tggagagccc
      361 tgagaatgcc tttgggctgc ctgaggatga gttcctggtg gtgctgcgct gtgtggtggc
      421 cggcatgaac cacctgcggg agaacggcat tgtgcatcgc gacatcaagc cggggaacat
      481 catgcgcctc gtaggggagg aggggcagag catctacaag ctgacagact tcggcgctgc
      541 ccgggagctg gatgatgatg agaagttcgt ctcggtctat gggactgagg agtacctgca
      601 tcccgacatg tatgagcggg cggtgcttcg aaagccccag caaaaagcgt tcggggtgac
      661 tgtggatctc tggagcattg gagtgacctt gtaccatgca gccactggca gcctgccctt
      721 catccccttt
//
