LOCUS       sequence-60949-e       10939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 10939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-EF1a-CHER-WPRE
FEATURES             Location/Qualifiers
     source          1..10939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        2784..3967
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3015..3959
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3916..3936
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3989..3998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3995..4705
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4140..4158)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4408..4427
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4796..5384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4849..4869)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5267..5278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6292..6516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6649..6668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7689..7706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7860..8448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7940..7959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8619..9410,9411..9479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9242..9261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9480..9584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9652..9670
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9721..9743)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9829..9967
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9882..9901
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattact aaaaattcna aaattttcgg gtttattaca gggacagcag agatccactt
     2521 tgggcggccg ctttggagct aagccagcaa tggtagaggg aagattctgc acgtcccttc
     2581 caggcggcct ccccgtcacc acccccccca acccgccccg accggagctg agagtaattc
     2641 atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa actcccacta
     2701 acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct cgtcatcact
     2761 gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2821 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     2881 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2941 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3001 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3061 atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat
     3121 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3181 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     3241 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3301 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     3361 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     3421 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3481 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3541 gcggcaacag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3601 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3661 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3721 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3781 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3841 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3901 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3961 tgtcgtggcg gccgctagca aggatcccgc caccatggtg agcaagggcg aggaggataa
     4021 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4081 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4141 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     4201 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4261 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4321 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4381 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4441 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4501 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4561 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4621 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4681 cggcatggac gagctgtaca agtaagaatt cgaagcttga tccggctgct aacaaagccc
     4741 gacgtacgac gcgtgctagc atgcctaggt cgacccggga tccgcggact agtataatca
     4801 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4861 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4921 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4981 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5041 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5101 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5161 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5221 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5281 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5341 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     5401 actagtgaat tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
     5461 taataataac cgggcgaggg ggatctcgac tctagaagct tggtaccttt aagaccaatg
     5521 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5581 ctaattcact cccagtttcg agttggcgcg cctgggagct cacggggaca gcccccccca
     5641 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     5701 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
     5761 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     5821 agcctgcaga cacctggggg atacggggaa aaagctttag gctgaaagag agatttagaa
     5881 tgacaggcgc gcctgggagc tcacggggac agcccccccc aaagccccca gggatgtaat
     5941 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     6001 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     6061 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     6121 gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacaggcg cgccaactcg
     6181 aattcattgc agactttcac tgaaacgggt ctctctggtt agaccagatc tgagcctggg
     6241 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagctcg actgtgcctt
     6301 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6361 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6421 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6481 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6541 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6601 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6661 tcttcccttc ctttctcgcc acgttcgccg gctgtgcggg agaacggagt tctattatga
     6721 ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac ttagtgtgta
     6781 gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt tgcgccgagt
     6841 cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg ggggtgccag
     6901 ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc ttaggtttta
     6961 caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta gcctgtagct
     7021 gcatgactcc taaaccataa tttctttttt gttttttttt ttttattttt gagacagggt
     7081 ctcactctgt cacctaggct ggagtgcagt ggtgcaatca cagctcactg cagcctcaac
     7141 gtcgtaagct caagcgatcc tcccacctca gcctgcctgg tagctgagac tacaagcgac
     7201 gccccagtta atttttgtat ttttggtaga ggcagcgttt tgccgtgtgg ccctggctgg
     7261 tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc caaagtgctg ggacaaccgg
     7321 ggccagtcac tgcacctggc cctaaaccat aatttctaat cttttggcta atttgttagt
     7381 cctacaaagg cagtctagtc cccaggcaaa aagggggttt gtttcgggaa agggctgtta
     7441 ctgtctttgt ttcaaactat aaactaagtt cctcctaaac ttagttcggc ctacacccag
     7501 gaatgaacaa ggagagcttg gaggttagaa gcacgatgga attggttagg tcagatctct
     7561 ttcactgtct gagttataat tttgcaatgg tggttcaaag actgcccgct tctgacacca
     7621 gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggcgctct
     7681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8041 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8281 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9601 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     9661 atcacgaggc cctttcgtct tcaagaactg cctcgcgcgt ttcggtgatg acggtgaaaa
     9721 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9781 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     9841 ccagtcacgt agcgatagcg gagtgtactg gcttaactat gcggcatcag agcagattgt
     9901 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9961 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10021 ctcttcgcta ttacgccagc gcggggaggc agagattgca gtaagctgag atcgcagcac
    10081 tgcactccag cctgggcgac agagtaagac tctgtctcaa aaataaaata aataaatcaa
    10141 tcagatattc caatcttttc ctttatttat ttatttattt tctattttgg aaacacagtc
    10201 cttccttatt ccagaattac acatatattc tatttttctt tatatgctcc agtttttttt
    10261 agaccttcac ctgaaatgtg tgtatacaaa atctaggcca gtccagcaga gcctaaaggt
    10321 aaaaaataaa ataataaaaa ataaataaaa tctagctcac tccttcacat caaaatggag
    10381 atacagctgt tagcattaaa taccaaataa cccatcttgt cctcaataat tttaagcgcc
    10441 tctctccacc acatctaact cctgtcaaag gcatgtgccc cttccgggcg ctctgctgtg
    10501 ctgccaacca actggcatgt ggactctgca gggtccctaa ctgccaagcc ccacagtgtg
    10561 ccctgaggct gccccttcct tctagcggct gcccccactc ggctttgctt tccctagttt
    10621 cagttacttg cgttcagcca aggtctgaaa ctaggtgcgc acagagcggt aagactgcga
    10681 gagaaagaga ccagctttac agggggttta tcacagtgca ccctgacagt cgtcagcctc
    10741 acagggggtt tatcacattg caccctgaca gtcgtcagcc tcacaggggg tttatcacag
    10801 tgcaccctta caatcattcc atttgattca caattttttt agtctctact gtgcctaact
    10861 tgtaagttaa atttgatcag aggtgtgttc ccagagggga aaacagtata tacagggttc
    10921 agtactatcg catttcagg
//
