LOCUS       sequence-60953-e       10905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-RK-GFP-mIRBP156-CHER-WPRE
FEATURES             Location/Qualifiers
     source          1..10905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2368..2485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2856..2865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2862..2864,2865..2867,2868..3581)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2862..2864,2865..2867,2868..3578)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2907..2928)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3168..3187)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3515..3536
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3588..3640
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3955..3969
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      3976..3985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3982..4692
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4127..4145)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4395..4414
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4762..5350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4815..4835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5233..5244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6258..6482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6615..6634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7655..7672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7826..8414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7906..7925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8585..9376,9377..9445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9208..9227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9446..9550)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9618..9636
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9687..9709)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9795..9933
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9848..9867
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2521 ggcgcccgcg cgctaccgga ctcagatctc gaggggcccc agaagcctgg tggttgtttg
     2581 tccttctcag gggaaaagtg aggcggcccc ttggaggaag gggccgggca gaatgatcta
     2641 atcggattcc aagcagctca ggggattgtc tttttctagc accttcttgc cactcctaag
     2701 cgtcctccgt gaccccggct gggatttagc ctggtgctgt gtcagccccg gtctcccagg
     2761 ggcttcccag tggtccccag gaaccctcga cagggcccgg tctctctcgt ccagcaaggg
     2821 cagggacggg ccacaggcca agggcggtac cggtcgccac catggtgagc aagggcgagg
     2881 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2941 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3001 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3061 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3121 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3181 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3241 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3301 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3361 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3421 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3481 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3541 ccgccgggat cactctcggc atggacgagc tgtacaagta agatatcctc gagctcaagc
     3601 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc ctacacagac atggctcagc
     3661 aagatgagat gcaatgttcc ttccgggtca aaccaccctg gcctcttctt cagagtccag
     3721 ctcatgtgct tgaattagac aggattaaag gcttagcaga gctggaagcc tcacatctaa
     3781 ctcccacatt gagccccaga ccttctgtct gcctgctaag aagggcaagg gagagaggca
     3841 gctgcaggac cacagccttg tacacacgga gctcttatcc aggagccaag gctctgggat
     3901 ccaccggtcg ccgctagcgt cgatcgaccc gggatcgata gtttaaacct gtacgacgat
     3961 gacgataagg atcccgccac catggtgagc aagggcgagg aggataacat ggccatcatc
     4021 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4081 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4141 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4201 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4261 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4321 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4381 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4441 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4501 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4561 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4621 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4681 ctgtacaagt aagaattcga agcttgatcc ggctgctaac aaagcccgaa aggagtcgac
     4741 ccgggatccg cggactagta taatcaacct ctggattaca aaatttgtga aagattgact
     4801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5341 gcctccccgc atcgataccg tcgaccacta gtgaattcca gctgagcgcc ggtcgctacc
     5401 attaccagtt ggtctggtgt caaaaataat aataaccggg cgagggggat ctcgactcta
     5461 gaagcttggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5521 taaaagaaaa ggggggactg gaagggctaa ttcactccca gtttcgagtt ggcgcgcctg
     5581 ggagctcacg gggacagccc cccccaaagc ccccagggat gtaattacgt ccctcccccg
     5641 ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc
     5701 ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc
     5761 ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaag
     5821 ctttaggctg aaagagagat ttagaatgac aggcgcgcct gggagctcac ggggacagcc
     5881 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
     5941 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
     6001 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
     6061 ctctttgagc ctgcagacac ctgggggata cggggaaaaa gctttaggct gaaagagaga
     6121 tttagaatga caggcgcgcc aactcgaatt cattgcagac tttcactgaa acgggtctct
     6181 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6241 gcctcaataa agctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6301 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6361 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6421 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6481 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctg
     6661 tgcgggagaa cggagttcta ttatgactca aatcagtctc cccaagcatt cggggatcag
     6721 agtttttaag gataacttag tgtgtagggg gccagtgagt tggagatgaa agcgtaggga
     6781 gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg gggccacaag atcggatgag
     6841 ccagtttatc aatccggggg tgccagctga tccatggagt gcagggtctg caaaatatct
     6901 caagcactga ttgatcttag gttttacaat agtgatgtta ccccaggaac aatttgggga
     6961 aggtcagaat cttgtagcct gtagctgcat gactcctaaa ccataatttc ttttttgttt
     7021 tttttttttt atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg
     7081 caatcacagc tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct
     7141 gcctggtagc tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca
     7201 gcgttttgcc gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca
     7261 gcctcccaaa gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt
     7321 tctaatcttt tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg
     7381 gggtttgttt cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc
     7441 ctaaacttag ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac
     7501 gatggaattg gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt
     7561 tcaaagactg cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg
     7621 agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     8041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9601 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaactgcctc
     9661 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9721 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9781 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtactggctt
     9841 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9901 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcgcgg ggaggcagag
    10021 attgcagtaa gctgagatcg cagcactgca ctccagcctg ggcgacagag taagactctg
    10081 tctcaaaaat aaaataaata aatcaatcag atattccaat cttttccttt atttatttat
    10141 ttattttcta ttttggaaac acagtccttc cttattccag aattacacat atattctatt
    10201 tttctttata tgctccagtt ttttttagac cttcacctga aatgtgtgta tacaaaatct
    10261 aggccagtcc agcagagcct aaaggtaaaa aataaaataa taaaaaataa ataaaatcta
    10321 gctcactcct tcacatcaaa atggagatac agctgttagc attaaatacc aaataaccca
    10381 tcttgtcctc aataatttta agcgcctctc tccaccacat ctaactcctg tcaaaggcat
    10441 gtgccccttc cgggcgctct gctgtgctgc caaccaactg gcatgtggac tctgcagggt
    10501 ccctaactgc caagccccac agtgtgccct gaggctgccc cttccttcta gcggctgccc
    10561 ccactcggct ttgctttccc tagtttcagt tacttgcgtt cagccaaggt ctgaaactag
    10621 gtgcgcacag agcggtaaga ctgcgagaga aagagaccag ctttacaggg ggtttatcac
    10681 agtgcaccct gacagtcgtc agcctcacag ggggtttatc acattgcacc ctgacagtcg
    10741 tcagcctcac agggggttta tcacagtgca cccttacaat cattccattt gattcacaat
    10801 ttttttagtc tctactgtgc ctaacttgta agttaaattt gatcagaggt gtgttcccag
    10861 aggggaaaac agtatataca gggttcagta ctatcgcatt tcagg
//
