LOCUS       sequence-60960-e       11676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Verrier JD, Madorsky I, Coggin WE, Geesey M, Hochman M, Walling E, 
            Daroszewski D, Eccles KS, Ludlow R, Semple-Rowland SL
  TITLE     Bicistronic lentiviruses containing a viral 2A cleavage sequence 
            reliably co-express two proteins and restore vision to an animal 
            model of LCA1.
  JOURNAL   PLoS One. 2011;6(5):e20553. doi: 10.1371/journal.pone.0020553. Epub 
            2011 May 27.
  PUBMED    21647387
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5):e20553. doi"; pages: " 
            10.1371/journal.pone.0020553. Epub 2011 May 27"
COMMENT     Sequence Label: pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
FEATURES             Location/Qualifiers
     source          1..11676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     236..256
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1877..2110
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2372..2489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2786..3969
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3017..3961
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3918..3938
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3989..4702
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4730..4786
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4787..5494
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4932..4950)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5200..5219
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5495..5521
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5533..6121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5586..5606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6004..6015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7029..7253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7386..7405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(8426..8443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8597..9185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8677..8696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9356..10147,10148..10216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9979..9998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10217..10321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10389..10407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10458..10480)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10566..10704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10619..10638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggccggagta tctagatgga gttccgcgtt acataactta
       61 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      121 ctgatgttcc catagtaacg ccaataggga ctttccattg acgctaatgg gagtttgttt
      181 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
      241 atgggcggta ggcgtgtact ctagaaggtc tatataagca gagctcgttt agtgaaccgt
      301 ttatactact tatctggtct ctctggttag accagatctg agcctgggag ctctctggct
      361 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctc aaagtagtgt
      421 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      481 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaagtaa agccagagga
      541 gatctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      601 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      661 gagcgtcggt attaagcggg ggagaattag ataaatggga aaaaattcgg ttaaggccag
      721 ggggaaagaa acaatataaa ctaaaacata tagtatgggc aagcagggag ctagaacgat
      781 tcgcagttaa tcctggcctt ttagagacat cagaaggctg tagacaaata ctgggacagc
      841 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acaatagcag
      901 tcctctattg tgtgcatcaa aggatagatg taaaagacac caaggaagcc ttagataaga
      961 tagaggaaga gcaaaacaaa agtaagaaaa aggcacagca agcagcagct gacacaggaa
     1021 acaacagcca ggtcagccaa aattacccta tagtgcagaa cctccagggg caaatggtac
     1081 atcaggccat atcacctaga actttaaatg catgggtaaa agtagtagaa gagaaggctt
     1141 tcagcccaga agtaataccc atgttttcag cattatcaga aggagccacc ccacaagatt
     1201 taaataccat gctaaacaca gtggggggac atcaagcagc catgcaaatg ttaaaagaga
     1261 ccatcaatga ggaagctgca gaatgggata gattgcatcc agtgcatgca gggcctattg
     1321 caccaggcca gatgagagaa ccaaggggaa gtgacatagc aggaactact agcaaattaa
     1381 gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga ggggacccag
     1441 aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat tcaacacaac
     1501 tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat aacactgaag
     1561 gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg tggcaggaag
     1621 taggaaaagc aatgtatgcc cctcccatca gtggacaaat tagatgttca tcaaatatta
     1681 ctgggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag atcttcagac
     1741 ctggaggagg cgatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1801 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1861 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1921 tgggcgcacg gtcaatgacg ctgacggtac aggccagaca attattgtct gatatagtgc
     1981 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     2041 tctggggcat caaacagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     2101 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     2161 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaataacatg acctggatgg
     2221 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     2281 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcttgcggcc
     2341 ggaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2401 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2461 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     2521 tttgggcggc cgctttggag ctaagccagc aatggtagag ggaagattct gcacgtccct
     2581 tccaggcggc ctccccgtca ccaccccccc caacccgccc cgaccggagc tgagagtaat
     2641 tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac
     2701 taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca
     2761 ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
     2821 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2881 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2941 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3001 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     3061 ttatggccct tgcgtgcctt gaattacttc cacgcccctg gctgcagtac gtgattcttg
     3121 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     3181 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3241 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3301 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3361 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3421 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3481 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3541 gggcggcaac aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     3601 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     3661 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     3721 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     3781 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     3841 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     3901 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     3961 ggtgtcgtgg cggccgctag ccgccaccat gagcaagggc gaggaactgt tcactggcgt
     4021 ggtcccaatt ctcgtggaac tggatggcga tgtgaatggg cacaaatttt ctgtcagcgg
     4081 agagggtgaa ggtgatgcca catacggaaa gctcaccctg aaattcatct gcaccactgg
     4141 aaagctccct gtgccatggc caacactggt cactaccctg acctatggcg tgcagtgctt
     4201 ttccagatac ccagaccata tgaagcagca tgactttttc aagagcgcca tgcccgaggg
     4261 ctatgtgcag gagagaacca tctttttcaa agatgacggg aactacaaga cccgcgctga
     4321 agtcaagttc gaaggtgaca ccctggtgaa tagaatcgag ctgaagggca ttgactttaa
     4381 ggaggatgga aacattctcg gccacaagct ggaatacaac tataactccc acaatgtgta
     4441 catcatggcc gacaagcaaa agaatggcat caaggtcaac ttcaagatca gacacaacat
     4501 tgaggatgga tccgtgcagc tggccgacca ttatcaacag aacactccaa tcggcgacgg
     4561 ccctgtgctc ctcccagaca accattacct gtccacccag tctgccctgt ctaaagatcc
     4621 caacgaaaag agagaccaca tggtcctgct ggagtttgtg accgctgctg ggatcacaca
     4681 tggcatggac gagctgtaca aggaattccc gggtcgactc ggatccggag ccacgaactt
     4741 ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcctatgg tgagcaaggg
     4801 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4861 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4921 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     4981 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5041 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5101 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5161 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5221 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5281 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5341 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5401 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5461 ccactccacc ggcggcatgg acgagctgta caagtaccca tacgacgtcc cagactacgc
     5521 ttaaactagt ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5581 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5641 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5701 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5761 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5821 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5881 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5941 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6001 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6061 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6121 catcgatacc gtcgaccact agtgaattcc agctgagcgc cggtcgctac cattaccagt
     6181 tggtctggtg tcaaaaataa taataaccgg gcgaggggga tctcgactct agaagcttgg
     6241 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6301 aggggggact ggaagggcta attcactccc agtttcgagt tggcgcgcct gggagctcac
     6361 ggggacagcc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
     6421 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
     6481 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
     6541 cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaaa gctttaggct
     6601 gaaagagaga tttagaatga caggcgcgcc tgggagctca cggggacagc cccccccaaa
     6661 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     6721 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     6781 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     6841 cctgcagaca cctgggggat acggggaaaa agctttaggc tgaaagagag atttagaatg
     6901 acaggcgcgc caactcgaat tcattgcaga ctttcactga aacgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct gtgcgggaga
     7441 acggagttct attatgactc aaatcagtct ccccaagcat tcggggatca gagtttttaa
     7501 ggataactta gtgtgtaggg ggccagtgag ttggagatga aagcgtaggg agtcgaaggt
     7561 gtccttttgc gccgagtcag ttcctgggtg ggggccacaa gatcggatga gccagtttat
     7621 caatccgggg gtgccagctg atccatggag tgcagggtct gcaaaatatc tcaagcactg
     7681 attgatctta ggttttacaa tagtgatgtt accccaggaa caatttgggg aaggtcagaa
     7741 tcttgtagcc tgtagctgca tgactcctaa accataattt cttttttgtt tttttttttt
     7801 tatttttgag acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag
     7861 ctcactgcag cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag
     7921 ctgagactac aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc
     7981 cgtgtggccc tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa
     8041 agtgctggga caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt
     8101 ttggctaatt tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt
     8161 tcgggaaagg gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta
     8221 gttcggccta cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt
     8281 ggttaggtca gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact
     8341 gcccgcttct gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8401 ttgcgtattg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     8821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     9061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10321 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10381 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaactgcct cgcgcgtttc
    10441 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10501 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10561 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtactggct taactatgcg
    10621 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    10681 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
    10741 gggcgatcgg tgcgggcctc ttcgctatta cgccagcgcg gggaggcaga gattgcagta
    10801 agctgagatc gcagcactgc actccagcct gggcgacaga gtaagactct gtctcaaaaa
    10861 taaaataaat aaatcaatca gatattccaa tcttttcctt tatttattta tttattttct
    10921 attttggaaa cacagtcctt ccttattcca gaattacaca tatattctat ttttctttat
    10981 atgctccagt tttttttaga ccttcacctg aaatgtgtgt atacaaaatc taggccagtc
    11041 cagcagagcc taaaggtaaa aaataaaata ataaaaaata aataaaatct agctcactcc
    11101 ttcacatcaa aatggagata cagctgttag cattaaatac caaataaccc atcttgtcct
    11161 caataatttt aagcgcctct ctccaccaca tctaactcct gtcaaaggca tgtgcccctt
    11221 ccgggcgctc tgctgtgctg ccaaccaact ggcatgtgga ctctgcaggg tccctaactg
    11281 ccaagcccca cagtgtgccc tgaggctgcc ccttccttct agcggctgcc cccactcggc
    11341 tttgctttcc ctagtttcag ttacttgcgt tcagccaagg tctgaaacta ggtgcgcaca
    11401 gagcggtaag actgcgagag aaagagacca gctttacagg gggtttatca cagtgcaccc
    11461 tgacagtcgt cagcctcaca gggggtttat cacattgcac cctgacagtc gtcagcctca
    11521 cagggggttt atcacagtgc acccttacaa tcattccatt tgattcacaa tttttttagt
    11581 ctctactgtg cctaacttgt aagttaaatt tgatcagagg tgtgttccca gaggggaaaa
    11641 cagtatatac agggttcagt actatcgcat ttcagg
//
