LOCUS       sequence-60984-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-AK1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             155..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gaaacatcac catcaccatc accatagcga
      181 ttacgacatc cccactactg agaatcttta ttttcaggga tccgaagaga agctgaagaa
      241 aaccaagatc atctttgtgg tgggtgggcc tggctcaggg aagggcaccc agtgtgagaa
      301 gatcgtgcag aagtatggct acacccacct ctccaccggg gacctcctgc ggtccgaggt
      361 cagctcaggc tcggccaggg gcaagaagct gtcggaaatc atggagaagg ggcagctggt
      421 tccactggag acagtgttgg acatgctccg ggatgccatg gtggccaaag tcaatacttc
      481 caaaggcttc ctgattgatg gctacccgcg ggaggtgcag caaggagaag agtttgagcg
      541 acggattgga cagcccacac tgctgctgta tgtggacgca ggccctgaga ccatgaccca
      601 gcggctcttg aaacgtggag agaccagcgg gcgtgtggac gacaatgagg agaccatcaa
      661 aaagcggctg gagacctatt acaaggccac agaacccgtc atcgccttct atgagaaacg
      721 tggcattgtg cgcaaggtca acgctgaggg ctccgtggac agtgtcttct cccaggtctg
      781 cacccacctg gacgccctaa
//
