LOCUS       sequence-61048-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-MPP6
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(43..57,58..63,64..80,81..87)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    63..79
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             130..141
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             154..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             199..219
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataggcgta tcacgaggcc ctttcgtctt cacctcgaga aatcataaaa aatttatttg
       61 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca atttcacaca
      121 gaattcatta aagaggagaa attaactatg aaacatcacc atcaccatca ccatagcgat
      181 tacgacatcc ccactactga gaatctttat tttcagggat cccagcaagt cttggaaaac
      241 cttacggagc tgccctcgtc tactggagca gaagaaatag acctaatttt cctcaaggga
      301 attatggaga atcctattgt aaaatcactt gctaaggctc atgagaggct agaagattcc
      361 aaactagaag ctgtcagtga caataacttg gaattagtca atgaaattct tgaagacatc
      421 actcctctaa taaatgtgga tgaaaatgtg gcagaattgg ttggtatact caaagaacct
      481 cacttccagt cactgttgga ggcccatgat attgtggcat caaagtgtta tgattcacct
      541 ccatcaagcc cagaaatgaa taattcttct atcaataatc agttattacc agtagatgcc
      601 attcgtattc ttggtattca caaaagagct ggggaaccac tgggtgtgac atttagggtt
      661 gaaaataatg atctggtaat tgcccgaatc ctccatgggg gaatgataga tcgacaaggt
      721 ctacttcatg tgggagatat aattaaagaa gtcaatggcc atgaggttgg aaataatcca
      781 aaggaattac aagaattact
//
