LOCUS       sequence-61049-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQStrep2-GADD45G
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             161..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             668..691
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      714..808
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc accatcacgg
      181 atctactctg gaagaagtcc gcggccagga cacagttccg gaaagcacag ccaggatgca
      241 gggtgccggg aaagcgctgc atgagttgct gctgtcggcg cagcgtcagg gctgcctcac
      301 tgccggcgtc tacgagtcag ccaaagtctt gaacgtggac cccgacaatg tgaccttctg
      361 tgtgctggct gcgggtgagg aggacgaggg cgacatcgcg ctgcagatcc attttacgct
      421 gatccaggct ttctgctgcg agaacgacat cgacatagtg cgcgtgggcg atgtgcagcg
      481 gctggcggct atcgtgggcg ccggcgagga ggcgggtgcg ccgggcgacc tgcactgcat
      541 cctcatttcg aaccccaacg aggacgcctg gaaggatccc gccttggaga agctcagcct
      601 gttttgcgag gagagccgca gcgttaacga ctgggtgccc agcatcaccc tccccgaggc
      661 ggccgcttgg agccacccgc agttcgaaaa ataagcttaa ttagctgagc ttggactcct
      721 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      781 ccgccgggcg ttttttattg gtgagaatcc aagctagctt ggcgagattt tcaggagcta
      841 aggaagctaa aatggagaaa
//
