LOCUS       sequence-61061-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Geng X, Huang C, Qin Y, McCombs JE, Yuan Q, Harry BL, Palmer AE, Xia
            NS, Xue D
  TITLE     Hepatitis B virus X protein targets Bcl-2 proteins to increase 
            intracellular calcium, required for virus replication and cell death
            induction.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18471-6. doi: 
            10.1073/pnas.1204668109. Epub 2012 Oct 22.
  PUBMED    23091012
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204668109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-6- 6"; volume: "109"; issue: "45"; pages: "18471-6"
COMMENT     Sequence Label: pcDNA3.1-Flag HBx
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             71..94
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(620..637)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    626..737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 ggctagcatg gactacaagg atgacgatga caagatggct gctaggctgt actgccaact
      121 ggatccttcg cgggacgtcc tttgtttacg tcccgtcggc gctgaatccc gcggacgacc
      181 cctctcgggg ccgcttggga ctctctcgtc cccttctccg tctgccgttc cagccgacca
      241 cggggcgcac ctctctttac gcggtctccc cgtctgtgcc ttctcatctg ccggtccgtg
      301 tgcacttcgc ttcacctctg cacgttgcat ggagaccacc gtgaacgccc atcagatcct
      361 gcccaaggtc ttacataaga ggactcttgg actcccagca atgtcaacga ccgaccttga
      421 ggcctacttc aaggactgtg tgtttaagga ctgggaggag ctgggggagg agattaggtt
      481 aaaggtcttt gtattaggag gctgtaggca taaattggtc tgcgcaccag caccatgcaa
      541 ctttttcacc tctgcctaag atatccagca cagtggcggc cgctcgagtc tagagggccc
      601 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      661 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      721 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtgggg
//
