LOCUS       sequence-61065-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Geng X, Huang C, Qin Y, McCombs JE, Yuan Q, Harry BL, Palmer AE, Xia
            NS, Xue D
  TITLE     Hepatitis B virus X protein targets Bcl-2 proteins to increase 
            intracellular calcium, required for virus replication and cell death
            induction.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18471-6. doi: 
            10.1073/pnas.1204668109. Epub 2012 Oct 22.
  PUBMED    23091012
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204668109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-6- 6"; volume: "109"; issue: "45"; pages: "18471-6"
COMMENT     Sequence Label: pcDNA3.1-Flag HBx (G124L, I127A)
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..106
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(632..649)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    638..749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagca tggactacaa ggatgacgat gacaagatgg ctgctaggct
      121 gtactgccaa ctggatcctt cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc
      181 ccgcggacga cccctctcgg ggccgcttgg gactctctcg tccccttctc cgtctgccgt
      241 tccagccgac cacggggcgc acctctcttt acgcggtctc cccgtctgtg ccttctcatc
      301 tgccggtccg tgtgcacttc gcttcacctc tgcacgttgc atggagacca ccgtgaacgc
      361 ccatcagatc ctgcccaagg tcttacataa gaggactctt ggactcccag caatgtcaac
      421 gaccgacctt gaggcctact tcaaggactg tgtgtttaag gactgggaag agctgctcga
      481 ggaggctagg ttaaaggtct ttgtattagg aggctgtagg cataaattgg tctgcgcacc
      541 agcaccatgc aactttttca cctctgccta agatatccag cacagtggcg gccgctcgag
      601 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      661 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      721 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      781 gggg
//
