LOCUS       sequence-61084-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-LRPAP1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(46..60,61..66,67..83,84..90)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             133..144
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             157..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             202..222
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaaataggcg tatcacgaag gccctttcgt cttcacctcg agaaatcata aaaaatttat
       61 ttgctttgtg agcggataac aattataata gattcaattg tgagcggata acaatttcac
      121 acagaattca ttaaagagga gaaattaact atgaaacatc accatcacca tcaccatagc
      181 gattacgaca tccccactac tgagaatctt tattttcagg gatccgcgcc gcggagggtc
      241 aggtcgtttc tgcgcgggct cccggcgctg ctactgctgc tgctcttcct cgggccctgg
      301 cccgctgcga gccacggcgg caagtactcg cgggagaaga accagcccaa gccgtccccg
      361 aaacgcgagt ccggagagga gttccgcatg gagaagttga accagctgtg ggagaaggcc
      421 cagcgactgc atcttcctcc cgtgaggctg gccgagctcc acgctgatct gaagatacag
      481 gagagggacg aactcgcctg gaagaaacta aagcttgacg gcttggacga agatggggag
      541 aaggaagcga gactcatacg caacctcaat gtcatcttgg ccaagtatgg tctggacgga
      601 aagaaggacg ctcggcaggt gaccagcaac tccctcagtg gcacccagga agacgggctg
      661 gatgacccca ggctggaaaa gctgtggcac aaggcgaaga cctctgggaa attctccggc
      721 gaagaactgg acaagctctg gcgggagttc ctgcatcaca aagagaaagt tcacgagtac
      781 aacgtcctgc tggagaccct gagcaggacc gaagaaatcc acgagaacgt cattagccc
//
