LOCUS       sequence-61103-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-CUTC
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             162..179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgagaggatc gcatcaccat caccatcacg
      181 gatctaaaag gcagggggcc tcctctgagc gaaaacgagc gcggataccg tccgggaagg
      241 ccggagcagc aaatggattt ctcatggaag tttgtgttga ttcagtggaa tcagctgtga
      301 atgcagaaag aggaggtgct gatcggattg aattatgttc tggtttatca gaggggggaa
      361 ctacacccag catgggtgtc cttcaagtag tgaagcagag tgttcagatc ccagtttttg
      421 tgatgattcg gccacgggga ggtgattttt tgtattcaga tcgtgaaatt gaggtgatga
      481 aggctgacat tcgtcttgcc aagctttatg gtgctgatgg tttggttttt ggggcattga
      541 ctgaagatgg acacattgac aaagagctgt gtatgtccct tatggctatt tgccgccctc
      601 tgccagtcac tttccaccga gcctttgaca tggttcatga tccaatggca gctctggaga
      661 ccctcttaac cttgggattt gaacgcgtgt tgaccagtgg atgtgacagt tcagcattag
      721 aagggctacc cctaataaag cgactcattg agcaggcaaa aggcaggatt gtggtaatgc
      781 caggaggtgg tataacagac agaaatctac aaaggatcct tgagggt
//
