LOCUS       sequence-61107-e        4699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with mDsRed with SpHis5 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4699)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-mDsRed-SpHis5
FEATURES             Location/Qualifiers
     source          1..4699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..682
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     403..422
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      703..890
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(758..782)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            965..2165
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        965..1308
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1309..1962
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      1968..2165
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2269..2288)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2270..2288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2375..2392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2546..3134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2626..2645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3305..4096,4097..4165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3928..3947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4166..4270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4338..4356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4394..4416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4516..4535
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4616..4634
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4616..4633
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taacatggac aacaccgagg acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga
       61 gggctccgtg aacggccact acttcgagat cgagggcgag ggcgagggca agccctacga
      121 gggcacccag accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga
      181 catcctgtcc ccccagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      241 ccccgactac atgaagctgt ccttccccga gggcttcacc tgggagcgct ccatgaactt
      301 cgaggacggc ggcgtggtgg aggtgcagca ggactcctcc ctgcaggacg gcaccttcat
      361 ctacaaggtg aagttcaagg gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa
      421 gactgccggc tgggagccct ccaccgagaa gctgtacccc caggacggcg tgctgaaggg
      481 cgagatctcc cacgccctga agctgaagga cggcggccac tacacctgcg acttcaagac
      541 cgtgtacaag gccaagaagc ccgtgcagct gcccggcaac cactacgtgg actccaagct
      601 ggacatcacc aaccacaacg aggactacac cgtggtggag cagtacgagc acgccgaggc
      661 ccgccactcc ggctcccagt agggcgcgcc acttctaaat aagcgaattt cttatgattt
      721 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
      781 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
      841 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct
      901 agggataaca gggtaatata gatctgttta gcttgcctcg tccccgccgg gtcacccggc
      961 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
     1021 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
     1081 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
     1141 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
     1201 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
     1261 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg
     1321 gcttttgtag aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa
     1381 gctcccttac ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac
     1441 caaaagggag aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat
     1501 catgcactgg ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc
     1561 atcgatgatc atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag
     1621 gctatgggta actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa
     1681 gctctttcta gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga
     1741 ttaaagcgtg aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc
     1801 ttttcggtag cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat
     1861 catcgtgctg aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt
     1921 actggaagtt ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa
     1981 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     2041 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     2101 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     2161 tactgctgtc gattcgatac taacgccgcc atccagttta aacgagctcg aattcatcga
     2221 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     2281 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     2341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     2761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4321 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4381 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4441 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4501 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     4561 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     4621 ggtgacacta tagaacgcgg ccgccagctg aagcttcgta cgctgcaggt cgacggatcg
     4681 gtgacggtgc tggtttaat
//
