LOCUS       sequence-61109-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-C10orf83
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             155..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      704..798
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gaaacatcac catcaccatc accatagcga
      181 ttacgacatc cccactactg agaatcttta ttttcaggga tccaccctga caaaaggttc
      241 cttcacctac tccagtgggg aggaatatcg tggcgagtgg aaggagggcc gcaggcatgg
      301 ttttggtcaa ctgatgtttg cagatggtgg cacctacctg ggtcattttg agaatgggct
      361 ctttaatggc tttggggtat tgaccttctc agatggttca aggtatgagg gggagtttgc
      421 ccagggcaag tttaatggcg tcggagtctt cattcgatat gacaacatga cctttgaggg
      481 ggaatttaaa aatggcagag tagatggttt tggcctgctg actttccctg atggttctca
      541 tggaatcccc cgcaatgaag gtctctttga gaacaacaag ctgctgcgac gtgagaagtg
      601 ttctgccatt gttcagcggg cccagagcgc ctccaagtca gccagaaatc tcactgcctg
      661 agcggccgct cgacctgcag ccaagcttaa ttagctgagc ttggactcct gttgatagat
      721 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
      781 ttttttattg gtgagaatcc aagctagctt ggcgagattt tcaggagcta aggaagctaa
      841 aatgg
//
