LOCUS       sequence-61110-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-C9orf58
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             156..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             201..221
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      717..811
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat caccatagcg
      181 attacgacat ccccactact gagaatcttt attttcaggg atcctcgggc gagctcagca
      241 acaggttcca aggagggaag gcgttcggct tgctcaaagc ccggcaggag aggaggctgg
      301 ccgagatcaa ccgggagttt ctgtgtgacc agaagtacag tgatgaagag aaccttccag
      361 aaaagctcac agccttcaaa gagaagtaca tggagtttga cctgaacaat gaaggcgaga
      421 ttgacctgat gtctttaaag aggatgatgg agaagcttgg tgtccccaag acccacctgg
      481 agatgaagaa gatgatctca gaggtgacag gaggggtcag tgacactata tcctaccgag
      541 actttgtgaa catgatgctg gggaaacggt cggctgtcct caagttagtc atgatgtttg
      601 aaggaaaagc caacgagagc agccccaagc cagttggccc ccctccagag agagacattg
      661 ctagcctgcc ctgagcggcc gctcgacctg cagccaagct taattagctg agcttggact
      721 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      781 ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga ttttcaggag
      841 ctaaggaagc taaaatggag aa
//
