LOCUS       sequence-61121-e        4900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-yETSapphire-CaUra3
FEATURES             Location/Qualifiers
     source          1..4900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..721
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      742..929
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(797..821)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1004..1347
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2166..2363
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2470..2489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2471..2489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2576..2593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2747..3335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2827..2846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3506..4297,4298..4366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4129..4148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4367..4471)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4539..4557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4595..4617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4717..4736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4817..4835
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4817..4834
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taacatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
       61 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
      121 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
      181 cttagtcact actttttctt atggtgttat ggtttttgct agatacccag atcatatgaa
      241 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
      301 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
      361 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
      421 caaattggaa tacaacttta actctcacaa tgtttacatc atggctgaca aacaaaagaa
      481 tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc
      541 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
      601 ttacttatcc attcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
      661 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata
      721 aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
      781 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
      841 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
      901 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag
      961 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
     1021 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
     1081 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
     1141 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     1201 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
     1261 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
     1321 tcacatcaca tccgaacata aacaaccatg acagtcaaca ctaagaccta tagtgagaga
     1381 gcagaaactc atgcctcacc agtagcacag cgattatttc gattaatgga actgaagaaa
     1441 accaatttat gtgcatcaat tgacgttgat accactaagg aattccttga attaattgat
     1501 aaattaggtc cttatgtatg cttaatcaag actcatattg atataatcaa tgatttttcc
     1561 tatgaatcca ctattgaacc attattagaa ctttcacgta aacatcaatt tatgattttt
     1621 gaagatagaa aatttgctga tattggtaat accgtaaaga aacaatatat tggtggagtt
     1681 tataaaatta gtagttgggc agatattacc aatgctcatg gtgtcactgg gaatggagtg
     1741 gttgaaggat taaaacaggg agctaaagaa accaccacca accaagagcc aagagggtta
     1801 ttgatgttag ctgaattatc atcagtggga tcattagcat atggagaata ttctcaaaaa
     1861 actgttgaaa ttgctaaatc cgataaggaa tttgttattg gatttattgc ccaacgtgat
     1921 atgggtggcc aagaagaagg atttgattgg cttattatga cacctggagt tggattagat
     1981 gataaaggtg atggattagg acaacaatat agaactgttg atgaagttgt tagcactgga
     2041 actgatatta tcattgttgg tagaggattg tttggtaaag gaagagatcc agatattgaa
     2101 ggtaaaaggt atagaaatgc tggttggaat gcttatttga aaaagactgg ccaattataa
     2161 tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt
     2221 atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg
     2281 acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta
     2341 tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtgtcga
     2401 aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc
     2461 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     2521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2941 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3181 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4501 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4561 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4681 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4741 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     4801 gtatcataca catacgattt aggtgacact atagaacgcg gccgccagct gaagcttcgt
     4861 acgctgcagg tcgacggatc ggtgacggtg ctggtttaat
//
