LOCUS       sequence-61140-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Nematode antibiotic selection (G418)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRG02
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(828..848)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(828..846)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(867..883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(867..883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
       61 tatcgccttc ttgacgagtt cttctgagtc caattactct tcaacatccc tacatgctct
      121 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
      181 tgttttttag cttcttttaa gccacctcta acaatgaaat tgtgtagatt caaaaataga
      241 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
      301 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
      361 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
      421 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
      481 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
      541 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
      601 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
      661 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
      721 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
      781 ctgtttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
      841 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaa
//
