LOCUS       sequence-61146-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom 
            KA, Gould KL, McCollum D
  TITLE     A highly efficient multifunctional tandem affinity purification 
            approach applicable to diverse organisms.
  JOURNAL   Mol Cell Proteomics. 2012 Aug;11(8):501-11. doi: 
            10.1074/mcp.O111.016246. Epub 2012 Apr 3.
  PUBMED    22474084
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O111.016246"; journalName: "Mol Cell Proteomics"; date:
            "2012-08"; volume: "11"; issue: "8"; pages: "501-11"
COMMENT     Sequence Label: pcDNA4-TO-Hygromycin-sfGFP-MAP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(205..228)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(247..360)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(370..393)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ttcatccata ccatgcgtga tgcccgctgc ggttacgaac tccagcagaa ccatatgatc
       61 gcgtttctcg ttcggatctt tagacagaac gctttgcgtg ctcagatagt gattgtctgg
      121 cagcagaaca ggaccatcac cgattggagt gttttgctgg tagtgatcag ccagctgcac
      181 gctgccgccg ctgctagcgc tgcccttgtc gtcatcgtct ttgtagtcag cgctaccgcc
      241 gccggagggc tcccgctggc cctgagggtg gtgctccagc ctggcccgca gctgctccag
      301 ctcgccggcc aggccctcca ccacgtggcc gccccgccag ccggtggtct tctcgtccat
      361 ggcgctgccg tgatggtgat ggtgatggtg atgagcggac tcgaggctgc catcctccac
      421 gttgtggcga attttaaaat tcgctttaat gccatttttt tgtttatcgg cggtgatgta
      481 aacattgtgg ctgttaaaat tgtattccag cttatggccc aggatattgc cgtcttcttt
      541 aaagtcaatg cctttcagct caatgcggtt taccagggta tcgccttcaa atttcacttc
      601 cgcacgcgtt ttgtacgtgc cgtcatcctt aaaggaaatc gtgcgttcct gcacatagcc
      661 ttccggcatg gcggacttga agaagtcatg ctgcttcata tggtccggat aacgagcaaa
      721 gcactgaaca ccataagtca gcgtcgttac cagagtcggc caaggtaccg gcagtttacc
      781 agtagtacag atgaacttca gcgtcagttt accattagtt gcgtcacctt caccct
//
