LOCUS       sequence-61150-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-NOHMA
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             155..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gaaacatcac catcaccatc accatagcga
      181 ttacgacatc cccactactg agaatcttta ttttcaggga tccgccactg cccagttgca
      241 gaggactccc atgagtgcac tggtatttcc caataagata tcaactgaac accagtcttt
      301 ggtgttagtg aagaggcttc tagcagtttc agtatcctgt atcacgtatt tgaggggaat
      361 attcccagaa tgcgcttatg gaacaagata tctagatgat ctttgtgtca aaatactgag
      421 agaagataaa aattgcccag gatctacaca gttagtgaaa tggatgctag gatgttatga
      481 tgctttacag aaaaaatatg tatacacaaa cccagaagat cctcagacaa tttcagaatg
      541 ttaccaattc aaattcaaat acaccaataa tggaccactc atggacttca taagtaaaaa
      601 ccaaagcaac gaatctagca tgttgtctac tgacaccaag aaagcaagca ttctcctcat
      661 tcgcaagatt tatatcctaa tgcaaaatct ggggccttta cctaatgatg tttgtttgac
      721 catgaaactt ttttactatg atgaagttac acccccagat taccagcctc ccggttttaa
      781 ggatggtgat tgtgaaggag ttatatttga aggggaacct atgtatttaa atgtgg
//
