LOCUS       sequence-61153-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-STX10
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             156..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             201..221
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat caccatagcg
      181 attacgacat ccccactact gagaatcttt attttcaggg atcctctctc gaagacccct
      241 tttttgtagt ccgaggcgag gtgcagaagg cggtgaacac ggcccgcggg ctgtaccagc
      301 gctggtgcga gctcctgcag gaaagcgcgg cggtcggacg cgaggagctg gactggacga
      361 ccaatgagct gcggaatggc ctgcgcagca tcgagtggga cctcgaggac ctggaagaga
      421 ccatcggtat agtggaagcc aacccaggca agttcaagct cccagccggg gacctgcagg
      481 agagaaaggt gttcgtggag cggatgcgag aggcagtcca ggaaatgaag gaccatatgg
      541 tcagcccaac agccgtagca tttttggaga ggaataacag agagatactc gcaggcaagc
      601 cagctgccca gaagtcaccc agcgacctgc tggatgccag cgcagtctcg gccacatctc
      661 gctacatcga ggagcagcag gccacacagc agctgatcat ggatgaacag gatcaacagc
      721 tggagatggt gtctgggagc atccaggttc tgaagcacat gtccggccgc gttggagaag
      781 agctggacga gcagggcatc atgctggatg ccttcgccca agagatggac cacacccagt
      841 cccgcatgga cg
//
