LOCUS       sequence-61157-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQStrep2-TRAPPC3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             162..179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             732..755
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgagaggatc gcatcaccat caccatcacg
      181 gatcctcgag gcaggcgaac cgtggcaccg agagcaagaa aatgagctct gagctcttca
      241 ccctgaccta tggtgccctg gtcacccagc tatgtaagga ctatgaaaat gatgaagatg
      301 tgaataaaca gctggacaaa atgggcttta acattggagt ccggctgatt gaagatttct
      361 tggctcggtc aaatgttggg aggtgccatg actttcggga aactgcggat gtcattgcca
      421 aggtggcgtt caagatgtac ttgggcatca ctccaagcat tactaattgg agcccagctg
      481 gtgatgaatt ctccctcatt ttggaaaata accccttggt ggactttgtg gaacttcctg
      541 ataaccactc atcccttatt tattccaatc tcttgtgtgg ggtgttgcgg ggagctttgg
      601 agatggtcca gatggctgtg gaggccaagt ttgtccagga caccctgaaa ggagacggtg
      661 tgacagaaat ccggatgaga ttcatcaggc ggattgagga caatcttcca gctggagagg
      721 aagcggccgc ttggagccac ccgcagttcg aaaaataagc ttaattagct gagcttggac
      781 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
      841 gttgccgccg ggcgttttt
//
