LOCUS       sequence-61171-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQStrep2-PREI3
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             161..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc accatcacgg
      181 atccgtcatg gcggagggga cggcagtgct gaggcggaac aggccgggca ccaaggcgca
      241 ggatttctat aattggcctg atgaatcctt tgatgaaatg gacagtacac tagctgttca
      301 acagtatatt caacagaaca taagagcaga ttgctccaat attgacaaaa ttcttgaacc
      361 acctgaaggc caagatgaag gtgtgtggaa gtatgaacat ttaaggcagt tctgccttga
      421 gctaaatgga cttgctgtca aacttcagag tgaatgccat ccagatactt gcactcaaat
      481 gacagcaact gaacaatgga tttttctttg tgcagctcat aaaactccaa aagagtgtcc
      541 tgctatagac tatactagac acacacttga tggtgctgca tgtcttctga atagcaataa
      601 atattttccc agcagggtta gcataaagga atcatctgta gcgaaactag gatcagtatg
      661 ccgtaggatt tacagaatat tttcacatgc ttattttcat catcggcaga tatttgatga
      721 atatgaaaat gaaacatttt tgtgtcatcg gtttactaag tttgtgatga aatacaattt
      781 gatgtccaag gataacctga ttgtaccaat tttagaagag gaagtacaga attcagtt
//
