LOCUS       sequence-61175-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     In3.4:EGFP plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJMD-In3.4:EGFP
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(14..35)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(275..294)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     622..643
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(738..757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(739..757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    762..896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(786..805)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     840..859
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggnngtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
       61 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      121 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      181 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      241 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      301 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      361 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      421 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      481 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      541 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      601 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      661 tctcggcatg gacgagctgt acaagggcgg tggaagatct gggaattcaa ggcctctcga
      721 gcctctagat tctgcagccc tatagtgagt cgtattacgt agatccagac atgataagat
      781 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      841 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      901 acaacaattg cattcatttt atgtttcnnn nnnggggnan ggnngtgggg aggttttttc
      961 caactttata tacatanttg nannnntcac ngggcgtcnn ntttacggta ccatcgannn
     1021 atnnna
//
