LOCUS       sequence-61176-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQStrep2-ARPC3
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(45..59,60..65,66..82,83..89)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             162..179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             726..749
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa aaaatttatt
       61 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
      121 cagaattcat taaagaggag aaattaacta tgagaggatc gcatcaccat caccatcacg
      181 gatctccggc ttaccactct tctctcatgg atcctgatac caaactcatc ggaaacatgg
      241 cactgttgcc tatcagaagt caattcaaag gacctgcccc cagagagaca aaagatacag
      301 atattgtgga tgaagccatc tattacttca aggccaatgt cttcttcaaa aactatgaaa
      361 ttaagaatga agctgatagg accttgatat atataactct ctacatttct gaatgtctga
      421 agaaactgca aaagtgcaat tccaaaagcc aaggtgagaa agaaatgtat acgctgggaa
      481 tcactaattt tcccattcct ggagagcctg gttttccact taacgcaatt tatgccaaac
      541 ctgcaaacaa acaggaagat gaagtgatga gagcctattt acaacagcta aggcaagaga
      601 ctggactgag actttgtgag aaagttttcg accctcagaa tgataaaccc agcaagtggt
      661 ggacttgctt tgtgaagaga cagttcatga acaagagtct ttcaggacct ggacaggcgg
      721 ccgcttggag ccacccgcag ttcgaaaaat aagcttaatt agctgagctt ggactcctgt
      781 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      841 gccgggcgtt ttt
//
