LOCUS       sequence-61181-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQStrep2-PIR
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             161..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc accatcacgg
      181 atctgggtcc tccaagaaag ttactctctc agtgctcagc cgggagcagt cggaaggggt
      241 tggagcgagg gtccggagaa gcattggcag acccgagtta aaaaatctgg atccgttttt
      301 actgtttgat gaatttaaag gaggtagacc aggaggattt cctgatcatc cacatcgagg
      361 ttttgaaaca gtatcctacc tcctggaagg gggcagcatg gcccatgaag acttctgtgg
      421 acacactggt aaaatgaacc caggagattt gcagtggatg actgcgggcc ggggcattct
      481 gcacgctgag atgccttgct cagaggagcc agcccatggc ctacaactgt gggttaattt
      541 gaggagctca gagaagatgg tggagcctca gtaccaggaa ctgaaaagtg aagaaatccc
      601 taaacccagt aaggatggtg tgacagttgc tgtcatttct ggagaagccc tgggaataaa
      661 gtccaaggtt tacactcgca caccaacctt atatttggac ttcaaattgg acccaggagc
      721 caaacattcc caacctatcc ctaaagggtg gacaagcttc atttacacga tatctggaga
      781 tgtgtatatt gggcccgatg atgcacaaca aa
//
