LOCUS       sequence-61220-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   O'Neill PR, Karunarathne WK, Kalyanaraman V, Silvius JR, Gautam N
  TITLE     G-protein signaling leverages subunit-dependent membrane affinity to
            differentially control betagamma translocation to intracellular 
            membranes.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 
            10.1073/pnas.1205345109. Epub 2012 Dec 3.
  PUBMED    23213235
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1205345109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-18- 18"; volume: "109"; issue: "51"; pages: 
            "E3568-77"
COMMENT     Sequence Label: EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(318..339)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     667..686
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gactcgagcg gccgccactg tgctggatat ctgcagaatt ctcagaggag cgcacagaag
       61 aacgccgcct cccggaaggg gttctcggaa gtgggcacag gagtgatgag ggggtcctca
      121 caagcgtggg catcacagta tgtcatcagg tctgctgctg ccttggacac ctttatccgg
      181 cacaagctgg cctcaatctt aagctgttcc accatcttgc gtgcttgacc aatactcata
      241 gtgctgttaa cgggggtctc ccctttcatg gtacccttgt acagctcgtc catgccgaga
      301 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      361 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      421 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      481 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
      541 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      601 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      661 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      721 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
      781 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      841 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      901 gacacgctga
//
