LOCUS       sequence-61224-e       10397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Zhou W, Dosey TL, Biechele T, Moon RT, Horwitz MS, Ruohola-Baker H
  TITLE     Assessment of hypoxia inducible factor levels in cancer cell lines 
            upon hypoxic induction using a novel reporter construct.
  JOURNAL   PLoS One. 2011;6(11):e27460. doi: 10.1371/journal.pone.0027460. Epub
            2011 Nov 23.
  PUBMED    22132102
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27460. doi"; pages: " 
            10.1371/journal.pone.0027460. Epub 2011 Nov 23"
COMMENT     Sequence Label: HBR-6U
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2399..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2700..2825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3318..3551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3735..3779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4041..4158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4349..4380
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     intron          4454..5026
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5034..5053
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        5081..5099
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5115..5133
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5115..5132
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(5168..5170,5171..5173,5174..5884)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5213..5234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5474..5493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5821..5842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        7132..8341
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8178..8197
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      8380..8389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8386..9063
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(8516..8534)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8566..8586)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8784..8803
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8978..9001
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             9137..9370
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9443..9564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9480..9499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9534..9553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9583..9718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9751..9769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9779..9796)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9779..9795)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9788..9810)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9940..10395
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10027..10046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10237..10258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgga
     2401 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2461 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc
     2701 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2761 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     2821 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     2881 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     2941 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3001 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3061 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3121 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     3181 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3241 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3301 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3361 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     3421 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     3481 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     3541 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     3601 tggaatgcta gttggagtaa taaatctctg gaacagattg gaatcacacg acctggatgg
     3661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     3901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     3961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4021 acggatctcg acggtatcgg ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4081 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4141 acaaaaattc aaaattttat cgactcagaa cacgccgtgt acgtgcttca cgtgtacgtg
     4201 gcgatcgtgt acgtgctgtc tcacacagca ctctagacta ctcgtgtacg tgtacgacgt
     4261 gtacgtgggc tacgtgtacg tgctgtctca cacagcacga agcttcgtag caatcggacc
     4321 gacgacgccg ggctcgagat ctagactcta gagggtatat aatggaagct cgaattccag
     4381 cttggcattc cggtactgtt ggtaaaaact aggcttttgc aaaaagctcc ggatcgatcc
     4441 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
     4501 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
     4561 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
     4621 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
     4681 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
     4741 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
     4801 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
     4861 gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc
     4921 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     4981 gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     5041 gctggttatt gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg
     5101 cgaattcaag cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg
     5161 atccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5221 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5281 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     5341 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     5401 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5461 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5521 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5581 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     5641 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     5701 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5761 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5821 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5881 caagaccggt ggcgcgccac gcgtccgcgg actagtctcg agttaatcga gttaattaag
     5941 ctagcctagt gccatttgtt cagtggttcg tagggctttc ccccactgtt tggctttcag
     6001 ttatatggat gatgtggtat tgggggccaa gtctgtacag catcttgagt ccctttttac
     6061 cgctgttacc aattttcttt tgtctttggg tatacattta aaccctaaca aaacaaagag
     6121 atggggttac tctctaaatt ttatgggtta tgtcattgga tgttatgggt ccttgccaca
     6181 agaacacatc atacaaaaaa tcaaagaatg ttttagaaaa cttcctatta acaggcctat
     6241 tgattggaaa gtatgtcaac gaattgtggg tcttttgggt tttgctgccc cttttacaca
     6301 atgtggttat cctgcgttga tgcctttgta tgcatgtatt caatctaagc aggctttcac
     6361 tttctcgcca acttacaagg cctttctgtg taaacaatac ctgaaccttt accccgttgc
     6421 ccggcaacgg ccacctctgt gccaagtgtt tgctgacgca acccccactg gctggggctt
     6481 ggtcatgggc catcagcgca tgcgtggaac cttttcggct cctctgccga tccatactgc
     6541 ggaactccta gccgcttgtt ttgctcgcag caggtctgga gcaaacatta tcgggactga
     6601 taactctgtt gtcctatccc gcaaatatac atcgtttcca tggctgctag gctgtgctgc
     6661 caactggatc ctgcgcggga cgtcctttgt ttacgtcccg tcggcgctga atcctgcgga
     6721 cgacccttct cggggtcgct tgggactctc tcgtcccctt ctccgtctgc cgttccgacc
     6781 gaccacgggg cgcacctctc tttacgcgga ctccccgtct gtgccttctc atctgccgga
     6841 ccgtgtgcac ttcgcttcac ctctgcacgt cgcatggaga ccaccgtgaa cgcccaccaa
     6901 atattgccca aggtcttaca taagaggact cttggactct cagcaatgtc aacgaccgac
     6961 cttgaggcat acttcaaaga ctgtttgttt aaagactggg aggagttggg ggaggagatt
     7021 aggttaaagg tctttgtact aggaggctgt aggcataaat tggtctgcgc accagcacca
     7081 tgtatcacta gagcggggta cccacacatt ccacatccac cggtgtcgcg aggcctccgc
     7141 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     7201 acgaagggcg caggagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     7261 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     7321 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     7381 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     7441 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     7501 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     7561 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     7621 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     7681 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     7741 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     7801 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     7861 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatgcggtgc
     7921 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     7981 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     8041 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     8101 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     8161 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     8221 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     8281 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     8341 ctccggaaaa gctagcatta ggatccgaat tcaccggtcg ccaccatggc ctcctccgag
     8401 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     8461 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     8521 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     8581 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     8641 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     8701 accgtgaccc aggactcctc cctgcaggac ggctccttca tctacaaggt gaagttcatc
     8761 ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg ctgggaggcc
     8821 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg
     8881 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag
     8941 cccgtgcagc tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac
     9001 gaggactaca ccatcgtgga gcagtacgag cgcgccgagg gccgccacca cctgttcctg
     9061 tagcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9121 aagaaaaggg gggacttgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     9181 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9361 atctctagca gtagtagttc atgtcatctt attattcang tatttataac ttgcaaagaa
     9421 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     9481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9541 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     9601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     9781 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     9841 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     9901 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     9961 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    10021 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    10081 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    10141 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    10201 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    10261 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    10321 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    10381 taacgtttac aatttcc
//
