LOCUS       sequence-61231-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC
  TITLE     IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary 
            epithelial cell transformation.
  JOURNAL   Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. 
            Epub 2012 Sep 24.
  PUBMED    23007157
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00468-12"; journalName: "Mol Cell Biol"; date: 
            "2012-12"; volume: "32"; issue: "23"; pages: "4756-68"
COMMENT     Sequence Label: pWB-MF-IKKe
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..41
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSKPKDPSQR"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggggtcttc aaaatctaaa ccaaaggacc ccagccagcg ccggcgcagg atccgaggtt
       61 accttgacta caaagacgat gacgacaagc aattgacaag tttgtacaaa aaagttggca
      121 tgcagagcac agccaattac ctgtggcaca cagatgacct gctggggcag ggggccactg
      181 ccagtgtgta caaggcccgc aacaagaaat ccggagagct ggttgctgtg aaggtcttca
      241 acactaccag ctacctgcgg ccccgcgagg tgcaggtgag ggagtttgag gtcctgcgga
      301 agctgaacca ccagaacatc gtcaagctct ttgcggtgga ggagacgggc ggaagccggc
      361 agaaggtact ggtgatggag tactgctcca gtgggagcct gctgagtgtg ctggagagcc
      421 ctgagaatgc ctttgggctg cctgaggatg agttcctggt ggtgctgcgc tgtgtggtgg
      481 ccggcatgaa ccacctgcgg gagaacggca ttgtgcatcg cgacatcaag ccggggaaca
      541 tcatgcgcct cgtaggggag gaggggcaga gcatctacaa gctgacagac ttcggcgctg
      601 cccgggagct ggatgatgat gagaagttcg tctcggtcta tgggactgag gagtacctgc
      661 atcccgacat gtatgagcgg gcggtgcttc gaaagcccca gcaaaaagcg ttcggggtga
      721 ctgtggatct ctggagcatt ggagtgacct tgtaccatgc agccactggc agcctgccct
      781 tcatcccctt tggtgggcca cggcggaaca aggagatcat gtaccggat
//
