LOCUS       sequence-61248-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINMSYB
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    597..618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(633..638,639..656,657..663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     676..698
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     695..711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     695..711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(727..744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(727..743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(736..758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca tcacaccatg tacgcaacgc ttgaagaagc
      181 cattgacgct gcacgcgaag aatttcttgc agacaacccc ggcatcgacg ccgaagatgc
      241 gaatgtgcaa cagttcaatg cccaaaaata cgttttgcag gacggcgaca tcatgtggca
      301 agttgagttt tttgccgacg aaggggaaga aggtgaatgt ttacctatgc ttagcggtga
      361 agccgcgcaa agtgtttttg atggcgacta tgatgagata gagatacgcc aggagtggca
      421 ggaagagaat acattacatg aatgggacga gggggaattt cagcttgagc caccgctgga
      481 taccgaggaa ggacgcgcag cagctgatga gtgggatgaa cgtagcagcg gtctggaagt
      541 tctgtttcag ggtaccgtcg acccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      601 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      661 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      721 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      781 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aat
//
