LOCUS       sequence-61250-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINHALO7
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca tcacggatcc gaaatcggta ctggctttcc
      181 attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg atgttggtcc
      241 gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct acgtgtggcg
      301 caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc tgatcggtat
      361 gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc gcttcatgga
      421 tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg actggggctc
      481 cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat
      541 ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc gcgagacctt
      601 ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga acgtttttat
      661 cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga tggaccatta
      721 ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc caaacgagct
      781 gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca tggactggct
      841 gcaccag
//
