LOCUS       sequence-61256-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINS3C
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    516..537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(552..557,558..575,576..582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    590..606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     595..617
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     614..630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     614..630
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(646..663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(646..662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca tcacgggagc gatagcgaag tgaaccagga
      181 agcgaaaccg gaagttaaac cggaagtgaa accggaaacc catattaatc tgaaagttag
      241 cgacggcagc agcgaaatct tttttaaaat taaaaaaacc accccgctgc gtcgcctgat
      301 ggaagccttt gcgaaacgtc agggtaaaga aatggatagc ctgcgctttc tgtatgacgg
      361 catccgtatt caggccgatc agaccccgga agacctggat atggaagaca acgatattat
      421 tgaagcgcat cgcgaacaga tcagcagcgg tctggaagtt ctgtttcagg gtaccgtcga
      481 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      541 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      601 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt
      661 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      721 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      781 gcgcagcctg
//
