LOCUS       sequence-61257-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Bird LE
  TITLE     High throughput construction and small scale expression screening of
            multi-tag vectors in Escherichia coli.
  JOURNAL   Methods. 2011 Sep;55(1):29-37. Epub 2011 Aug 10.
  PUBMED    21856427
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2011-09"; volume: "55(1)"; pages: "29-37. Epub 2011 Aug 10"
COMMENT     Sequence Label: pOPINTF
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca tcaccaagtt tcagttgaaa ccactcaagg
      181 ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg ctgttaaaag
      241 cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag gcaaagtgcc
      301 aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc tgggtgacct
      361 gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg ctggcgcacc
      421 gacttatgtt ccgggcgaat acaagctggg tgaagacttc acttactctg tagagtttga
      481 agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa aaccgatcgt
      541 tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac agcaggcgac
      601 ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg acttcaccgg
      661 ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac tggcgatggg
      721 ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag ctggcgaaga
      781 gttcaccatc gacgtgacct tcccggaaga ataccacgca gaa
//
