LOCUS       sequence-61259-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    239..260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(275..280,281..298,299..305)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    313..329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     318..340
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     337..353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     337..353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             349..522
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(369..386)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(369..385)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..400)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             552..569
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(659..678)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    724..779
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(778..797)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc
      181 ccagccggcg atggtaccgt cgacccgact ggaaagcggg cagtgagcgc aacgcaatta
      241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      361 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      421 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      481 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcca agctttctag
      541 accagtttaa acatcaccat caccatcact aagtgattaa cctcaggtgc aggctgccta
      601 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatcgatc
      661 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
      721 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
      781 tcggaaggac atatgggagg gcaaatcatt taaa
//
